Enhanced detection of malignant glioma xenograft by fluorescein-human serum albumin conjugate
Enhanced detection of malignant glioma xenograft by fluorescein-human serum albumin conjugate
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DOI：
10.1023/b:neon.0000021783.62610.1b
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发表时间：
2004-03-01
影响因子：
3.9
通讯作者：
Kuroiwa, T
中科院分区：
文献类型：
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作者：
Ichioka, T;Miyatake, SI;Kuroiwa, T
对象：在恶性胶质瘤的手术切除中，明确肿瘤组织与正常脑组织的边界是非常重要的。为此，我们将荧光素和人血清白蛋白（FLS-HSA）偶联，并通过荧光显微镜比较其与单独的荧光素钠（FLS-Na）在检测SCID小鼠的人脑胶质瘤异种移植瘤中的有效性。制备皮下移植U251 MG细胞的SCID小鼠作为肿瘤模型。在静脉内施用FLS-HSA缀合物或单独的FLS-Na后15、30、60、180、360或720分钟处死动物（n = 3）。用数码相机拍摄荧光图像，并量化每个图像中肿瘤和周围组织的亮度。在接受FLS-Na的荷瘤小鼠组中，给药试剂后60 min肿瘤组织的荧光消失，并且在任何时间点肿瘤和周围组织之间的亮度均无显著差异。另一方面，注射FLS-HSA显示相对肿瘤选择性亮度和足够的对比度之间的肿瘤和周围组织administration.Conclusion：FLS-HSA的特异性和荧光持续性的优点，为识别异种皮下肿瘤移植模型中的胶质瘤结节的目的。
Object: During the surgical resection of malignant gliomas, it is important to make clear the border zone between the tumor tissue and normal brain tissue. For this purpose, we conjugated fluorescein and human serum albumin (FLS-HSA) and compared its effectiveness against that of fluorescein-sodium (FLS-Na) alone in detecting human glioma xenografts in SCID mice through a fluorescence microscope.Methods: We made FLS-HSA conjugate using carbodiimide as a linking reagent. SCID mice, with U251MG cells transplanted subcutaneously, were prepared as tumor models. The animals were sacrificed 15, 30, 60, 180, 360, or 720 min after the intravenous administration of either the FLS-HSA conjugate or FLS-Na alone (n = 3). Fluorescence images were taken with a digital camera, and the brightness of the tumor and that of the peripheral tissue in each image were quantified. In the group of tumor-bearing mice that received FLS-Na, the fluorescence of tumor tissue disappeared 60 min after the reagent was administered, and there was no significant difference in brightness between the tumor and peripheral tissue at any time point. On the other hand, injection of FLS-HSA revealed relative tumor-selective brightness and sufficient contrast between the tumor and surrounding tissue 60 and 360 min after administration.Conclusion: FLS-HSA has the advantages of specificity and persistence of fluorescence over FLS-Na for the purpose of identifying glioma nodules in xenogenic subcutaneous tumor transplantation models.