Investigation of the Association Between the Fecal Microbiota and Breast Cancer in Postmenopausal Women: a Population-Based Case-Control Pilot Study
Investigation of the Association Between the Fecal Microbiota and Breast Cancer in Postmenopausal Women: a Population-Based Case-Control Pilot Study
复制标题
DOI：
10.1093/jnci/djv147
复制
发表时间：
2015-08-01
影响因子：
10.3
通讯作者：
Feigelson, Heather Spencer
中科院分区：
文献类型：
--
作者：
Goedert, James J.;Jones, Gieira;Feigelson, Heather Spencer
我们调查了48名绝经后乳腺癌病例患者治疗前与48名对照患者的肠道微生物群是否不同。通过Illumina测序和16 S rRNA基因分类来确定粪便DNA中的微生物群谱。在尿液中定量测定雌激素。病例对照比较采用微生物群α多样性的线性和无条件逻辑回归（PD_全树）和β多样性的UniFrac分析，双侧统计检验。在对照组患者中，总雌激素与α多样性相关（斯皮尔曼Rho = 0.37，P = 0.009），但在病例组患者中则不相关（斯皮尔曼Rho = 0.04，P = 0.77）。与对照组患者相比，病例组患者的微生物群组成（β多样性，P = 0.006）和α多样性（P = 0.004）在统计学上显著改变。校正雌激素和其他协变量后，癌症的比值比为0.50（95%置信区间= 0.30至0.85）/α多样性三分位数。调整多重比较后，具体类群的差异没有统计学意义。这项初步研究表明，患有乳腺癌的绝经后妇女的肠道微生物群的组成和雌激素非依赖性低多样性发生了改变。这些因素是否影响乳腺癌的风险和预后尚不清楚。
We investigated whether the gut microbiota differed in 48 postmenopausal breast cancer case patients, pretreatment, vs 48 control patients. Microbiota profiles in fecal DNA were determined by Illumina sequencing and taxonomy of 16S rRNA genes. Estrogens were quantified in urine. Case-control comparisons employed linear and unconditional logistic regression of microbiota alpha-diversity (PD_whole tree) and UniFrac analysis of beta-diversity, with two-sided statistical tests. Total estrogens correlated with alpha-diversity in control patients (Spearman Rho = 0.37, P =.009) but not case patients (Spearman Rho = 0.04, P =.77). Compared with control patients, case patients had statistically significantly altered microbiota composition (beta-diversity, P =.006) and lower alpha-diversity (P =.004). Adjusted for estrogens and other covariates, odds ratio of cancer was 0.50 (95% confidence interval = 0.30 to 0.85) per alpha-diversity tertile. Differences in specific taxa were not statistically significant when adjusted for multiple comparisons. This pilot study shows that postmenopausal women with breast cancer have altered composition and estrogen-independent low diversity of their gut microbiota. Whether these affect breast cancer risk and prognosis is unknown.