Dodeca-CLE peptides as suppressors of plant stem cell differentiation
Dodeca-CLE peptides as suppressors of plant stem cell differentiation
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DOI：
10.1126/science.1128436
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发表时间：
2006-08-11
期刊：
影响因子：
56.9
通讯作者：
Fukuda, Hiroo
中科院分区：
文献类型：
--
作者：
Ito, Yasuko;Nakanomyo, Ikuko;Fukuda, Hiroo
在植物和动物中，在细胞间通讯中发出信号的小肽配体被认为是发育的重要组成部分。单细胞转分化的生物测定表明，具有两个羟脯氨酸残基的十二肽是CLE家族基因的功能产物，其中包括拟南芥中的CLAVATA 3。十二肽在浓度为10（-11）M时抑制木质部细胞发育并促进细胞分裂。一个应用程序，对应于所有26个拟南芥CLE蛋白家族成员，合成的十二肽揭示了两个抵消信号通路参与干细胞的命运。
In plants and animals, small peptide ligands that signal in cell-cell communication have been suggested to be a crucial component of development. A bioassay of single-cell transdifferentation demonstrates that a dodecapeptide with two hydroxyproline residues is the functional product of genes from the CLE family, which includes CLAVATA3 in Arabidopsis. The dodecapeptide suppresses xylem cell development at a concentration of 10(-11) M and promotes cell division. An application, corresponding to all 26 Arabidopsis CLE protein family members, of synthetic dodecapeptides reveals two counteracting signaling pathways involved in stem cell fate.