Cellular reprogramming in the F3 mouse with paternal F0 radiation history.
Cellular reprogramming in the F3 mouse with paternal F0 radiation history.
复制标题
具有父系 F0 辐射史的 F3 小鼠的细胞重编程。
DOI：
10.1080/095530002317577330
复制
发表时间：
2002
影响因子：
2.6
通讯作者：
Overstreet,JW
中科院分区：
文献类型：
--
作者：
Vance,MM;Baulch,JE;Raabe,OG;Wiley,LM;Overstreet,JW
PurposeIn the mouse, paternal F 0 acute irradiation of Type B spermatogonia produces biological effects in offspring, including altered signalling kinase activities and protein levels. It was hypothesized that these effects represented cellular reprogramming that would alter the response of somatic cells in these offspring to an acute ionizing radiation exposure.Materials and methodsNineteen-day-old third generation (F 3) CD1 mice with and without an acute 1.0 Gy paternal F 0 radiation history each received an acute dose of 1.0 Gy from attenuated 137 C γ-rays. Kidney PKC and MAPK activities, and p53 protein levels were evaluated immediately following F 3 irradiation. The same endpoints and DNA damage were evaluated in kidney-derived fibroblast primary cell cultures 3 weeks post-irradiation.ResultsKidneys had significantly decreased PKC and MAPK activities and p53 protein levels related to F 0 irradiation and increased PKC and MAPK activities following F 3 irradiation irrespective of F 0 radiation history. Kidney-derived fibroblasts had significant changes or strong trends for all selected endpoints based upon cross-interaction of F 0 radiation history with F 3 irradiation. Comet assays demonstrated significantly increased DNA damage in fibroblasts related to F 0 irradiation and increased DNA damage following F 3 irradiation. However, significantly decreased F 3 irradiation damage was demonstrated based upon cross-interaction of F 0 radiation.ConclusionsThe data suggest that irradiation of paternal F 0 Type B spermatogonia resulted in cellular reprogramming causing offspring with this radiation history to have altered responses to acute somatic γ-irradiation.
