Low oxygen tension enhances osteogenic potential of bone marrow-derived mesenchymal stem cells with osteonecrosis-related functional impairment.
Low oxygen tension enhances osteogenic potential of bone marrow-derived mesenchymal stem cells with osteonecrosis-related functional impairment.
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DOI：
10.1155/2015/950312
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发表时间：
2015
影响因子：
4.3
通讯作者：
Wang K
中科院分区：
文献类型：
--
作者：
Fan L;Liu R;Li J;Shi Z;Dang X;Wang K
目标。糖皮质激素会对骨髓间充质干细胞（BMMSCs）的功能产生不利影响，需要纠正这种异常的策略；我们评估了低氧（2%）对骨坏死兔骨髓间充质干细胞的影响。方法。将正常兔和骨坏死兔骨髓间充质干细胞分为4组：(1)正常-常氧组，正常骨髓间充质干细胞在20%氧下培养；(2)骨坏死-缺氧组，骨坏死兔骨髓间充质干细胞在20%氧气条件下培养；(3)骨坏死低氧处理组，骨坏死兔骨髓间充质干细胞在2%氧下培养；(4)正常-低氧处理组，正常骨髓间充质干细胞在2%氧下培养。研究了MSCs的增殖、成骨和成脂分化以及干细胞基因、成骨和成脂分化标志物的表达。结果。与正常家兔骨髓间充质干细胞相比，骨坏死家兔骨髓间充质干细胞的增殖能力明显降低，干性基因表达受到抑制，成骨细胞形成减少，脂肪细胞形成增加，提示骨坏死相关损伤。低氧（2%）处理的骨坏死兔骨髓间充质干细胞不仅增殖和成骨潜能增加，而且成脂潜能降低。结论。低氧（2%）培养是增强受糖皮质激素影响的骨髓间充质干细胞功能的一种新策略，对未来治疗股骨头坏死的策略具有重要意义。
Objective. Glucocorticoids can affect the function of bone marrow-derived mesenchymal stem cells (BMMSCs) adversely and merit the requirement for a strategy to correct this anomaly; we assessed the effect of low oxygen (2%) on BMMSCs from rabbits with osteonecrosis. Methods. Bone marrow-derived mesenchymal stem cells from normal rabbits and rabbits with osteonecrosis were divided into four groups: (1) normal-normoxia group, with normal BMMSCs cultured under 20% oxygen; (2) osteonecrosis-normoxia group, with BMMSCs from rabbits with osteonecrosis cultured under 20% oxygen; (3) osteonecrosis-low oxygen treated group, with BMMSCs from rabbits with osteonecrosis cultured under 2% oxygen; (4) normal-low oxygen treated group, with normal BMMSCs cultured under 2% oxygen. The proliferation, osteogenic, and adipogenic differentiation of MSCs and expression of stemness genes, osteogenic, and adipogenic differentiation markers were investigated. Results. Compared with BMMSCs from normal rabbits, those from osteonecrosis rabbits showed significantly reduced proliferation ability, repressed expression of stemness genes, decreased osteoblasts formation, and increased adipocytes formation, indicating an osteonecrosis-related impairment. Low oxygen (2%) treated BMMSCs from osteonecrosis rabbits showed not only increased proliferation and osteogenic potential but also decreased adipogenic potential. Conclusion. Low oxygen (2%) culture represents a novel strategy to augment BMMSC function affected by glucocorticoids and holds significance for future strategies to treat femoral head osteonecrosis.
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影响因子：
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