EFFECT OF BACTERIAL ENDOTOXINS ON WATER INTAKE AND BODY WEIGHT OF MICE
EFFECT OF BACTERIAL ENDOTOXINS ON WATER INTAKE AND BODY WEIGHT OF MICE
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DOI：
10.1084/jem.113.5.921
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发表时间：
1961-01-01
影响因子：
15.3
通讯作者：
SCHAEDLER, RW
中科院分区：
文献类型：
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作者：
DUBOS, RJ;SCHAEDLER, RW
将革兰氏阴性杆菌内毒素注射到 NCS 小鼠体内会导致这些动物的饮水量立即减少或中断，从而导致体重减轻。通过 3 种不同的技术从 4 种不同的革兰氏阴性杆菌培养物制备的内毒素产生的产物在抑制水摄入方面具有大致相同的活性。最小有效剂量为0.1μg或更少。对于所有测试的毒素制剂，效果的持续时间与注射的剂量直接相关。事实证明，热灭活的大肠杆菌细胞与从革兰氏阴性杆菌制备的内毒素大致相同。相比之下，热灭活的结核分枝杆菌（BCG）细胞的活性要低得多，而热灭活的金黄色葡萄球菌细胞则基本上没有活性。先前用内毒素处理的小鼠对正常动物随后用相同材料处理引起的水摄入抑制表现出显着程度的耐受性。也可以通过接种热灭活的革兰氏阴性杆菌来诱导耐受性。一种细菌的耐受性与另一种细菌的耐受性重叠，但对动物接种疫苗的细菌培养物制备的内毒素的耐受性更为明显。与饲喂缺乏饮食（玉米）的小鼠相比，饲喂完整饮食的小鼠内毒素对饮水量的抑制作用的持续时间要短得多。大约需要相同剂量的内毒素(0.1μg)来抑制水的摄入、减少多形核白细胞的流入并增强葡萄球菌感染。
Injection of endotoxin of gram-negative bacilli into NCS mice caused an immediate reduction or interruption of water intake by these animals, with a resultant loss of body weight. Endotoxins prepared by 3 different techniques from 4 different cultures of gram-negative bacilli yielded products having approximately the same activity in inhibiting water intake. The minimum effective dose was 0.1 [mu]g or less. With all toxin preparations tested, the duration of the effect was directly related to the dose injected. The heat-killed cells of Escherichia coli proved approximately as effective as the endotoxins prepared from gram-negative bacilli. In contrast, heat-killed cells of Myco-bacterium tuberculosis (BCG) were much less active, and heat-killed cells of Staphylococcus aureus were essentially inactive. Mice previously treated with endotoxin exhibited a marked degree of tolerance to the inhibition of water intake caused in normal animals by a subsequent treatment with the same material. Tolerance could also be induced by vaccination with heat-killed gram-negative bacilli. Tolerance overlapped from one bacterial species to another but was more pro-nounced toward the endotoxin prepared from the bacterial culture with which the animal was vaccinated. The duration of the inhibitory effect of endotoxin on water intake was much shorter with mice fed a complete diet than with mice fed a deficient diet (corn). It took approximately the same dose of endotoxin (0.1 [mu]g) to inhibit water intake, reduce the influx of polymorphonuclear leucocytes, and enhance staphylococcal infection.