Expression of ADAMTs-5 and TIMP-3 in the condylar cartilage of rats induced by experimentally created osteoarthritis
Expression of ADAMTs-5 and TIMP-3 in the condylar cartilage of rats induced by experimentally created osteoarthritis
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实验性骨关节炎大鼠髁突软骨中ADAMTs-5和TIMP-3的表达
DOI：
10.1016/j.archoralbio.2014.02.016
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发表时间：
2014-05-01
影响因子：
3
通讯作者：
Shi, Jiejun
中科院分区：
文献类型：
--
作者：
Li, Wen;Wu, Mengjie;Shi, Jiejun
目的：研究ADAMTs-5和TIMP-3在颞下颌关节骨关节炎（TMJOA）模型大鼠中的表达，探讨ADAMT5-5和TIMP-3参与早期OA降解的可能作用。设计：32只SD大鼠分为4组：2周对照组（NC1）、2周OA组（OA1）、4周对照组(NC2) 和 4 周 OA 组 (OA2)。每组有8只大鼠。注射胶原酶用于建立TMJOA模型。采用HE染色分析髁突软骨的结构变化。 Western blot和RT-PCR分别检测ADAMTs-5和TIMP-3蛋白和mRNA水平的表达。结果：HE分析显示NC1、NC2和OA1组未见明显变化，而OA2组出现轻度损伤。 NC1和OA1之间ADAMTs-5蛋白表达水平无显着差异，但4周组ADAMTs-5表达较NC2组显着增加。在mRNA水平上，2周和4周OA组ADAMTs-5的表达较匹配对照组显着增加。同时，TIMP-3的表达显着下降，呈现出完全不同的趋势。结论：ADAMTs-5和TIMP-3的表达在TMJOA早期发生显着变化，提示ADAMTs-5和TIMP-3可能在髁突软骨退化的初期发挥重要作用。 (C) 2014 Elsevier Ltd. 保留所有权利。
Objective: To study the expression of ADAMTs-5 and TIMP-3 in temporomandibular joint osteoarthritis (TMJOA) model rats, to explore and confer the possible effects of ADAMT5-5 and TIMP-3 involved in the degradation of the early stage of OA.Design: 32 SD rats were divided into four groups: 2-week control group (NC1), 2-week OA group (OA1), 4-week control group (NC2) and 4-week OA group (OA2). Each group had 8 rats. Injection of collagenase was used to build up the TMJOA model. HE staining was used to analyze the structural change of condyle cartilage. Western blot and RT-PCR were used to measure the expression of ADAMTs-5 and TIMP-3 in protein and mRNA levels respectively.Results: HE analysis revealed that no significant changes were observed in NC1, NC2 and OA1 groups, while mild damages appeared in OA2 group. No significant differences were achieved in the expression of ADAMTs-5 in protein levels between NC1 and OA1, but the expression of ADAMTs-5 in 4-week group increased significantly compared to that in the NC2 group. On mRNA level, the expression of ADAMTs-5 in 2-week and 4-week OA groups increased significantly compared to that in the matched control group. Meanwhile, the expression of TIMP-3 decreased significantly, showing a completely different trend.Conclusions: The expression of ADAMTs-5 and TIMP-3 changed significantly in the early stage of TMJOA, which indicated that ADAMTs-5 and TIMP-3 may be play an important part in the initial stage of condylar cartilage degradation. (C) 2014 Elsevier Ltd. All rights reserved.