Isolation and characterization of the Rickettsia prowazekii recA gene.
Isolation and characterization of the Rickettsia prowazekii recA gene.
复制标题
普瓦泽基立克次体recA 基因的分离和表征。
DOI：
10.1128/jb.176.6.1777-1781.1994
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发表时间：
1994
影响因子：
3.2
通讯作者：
Wood,DO
中科院分区：
文献类型：
--
作者：
Dunkin,SM;Wood,DO
RecA基因是从普氏立克次体胞内专性细菌中分离得到的。Prowazekii RECA与大肠杆菌RECA的氨基酸序列比较表明，62%的残基是相同的。与嗜肺军团菌RecA的同源性最高，有69%的残基完全一致。E.coliRecA与功能活性相关的氨基酸残基在立克次体RecA中保守，Prowazekii rrecA基因补充了E.colirecA突变体对紫外光和甲烷磺酸甲酯的敏感性以及重组缺陷。立克次体recA上游的特征区域不包含与E.coliLexA结合位点(SOS Box)同源的序列，这表明普氏立克次体recA基因的调控存在差异。
The recA gene has been isolated from Rickettsia prowazekii, an obligate intracellular bacterium. Comparison of the amino acid sequence of R. prowazekii RecA with that of Escherichia coli RecA revealed that 62% of the residues were identical. The highest identity was found with RecA of Legionella pneumophila, in which 69% of the residues were identical. Amino acid residues of E. coli RecA associated with functional activities are conserved in rickettsial RecA, and the R. prowazekii recA gene complements E. coli recA mutants for UV light and methyl methanesulfonate sensitivities as well as recombinational deficiencies. The characterized region upstream of rickettsial recA did not contain a sequence homologous to an E. coli LexA binding site (SOS box), suggesting differences in the regulation of the R. prowazekii recA gene.