Cell type and distribution in the porcine temporomandibular joint disc
Cell type and distribution in the porcine temporomandibular joint disc
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DOI：
10.1016/j.joms.2005.10.009
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发表时间：
2006-02-01
影响因子：
1.9
通讯作者：
Athanasiou, KA
中科院分区：
文献类型：
--
作者：
Detamore, MS;Hegde, JN;Athanasiou, KA
目的：令人惊讶的是，人们对颞下颌关节（TMJ）盘的细胞组成知之甚少，而这在组织工程研究中是一个关键部分。为此，对颞下颌关节盘中的细胞类型进行了区域鉴定和定量分析。
材料与方法：通过组织学、电子显微镜和免疫组织化学方法对猪的颞下颌关节盘进行检查。组织学采用苏木精 - 伊红染色来确定细胞类型和细胞数量的区域差异。透射电子显微镜用于阐明颞下颌关节盘细胞与透明软骨的软骨细胞在细胞器含量和细胞周基质方面的差异。免疫组织化学用于评估颞下颌关节盘中平滑肌和骨骼肌特性的存在情况。
结果：颞下颌关节盘中成纤维细胞与类软骨细胞的总体比例约为2.35∶1，中间区域类软骨细胞的相对数量最高。电子显微镜显示颞下颌关节盘类软骨细胞与透明软骨的软骨细胞在细胞器和细胞周区域方面存在明显差异。免疫染色发现血管形式的平滑肌，在前带最为显著。未观察到骨骼肌。
结论：颞下颌关节盘的细胞与透明软骨的细胞明显不同，因此不应将其称为软骨细胞。颞下颌关节盘细胞由分布不均的亚群组成，其中成纤维细胞多于纤维软骨细胞。（c）2006年美国口腔颌面外科医师协会
Purpose: Surprisingly little is known about the cellular composition of the temporomandibular joint (TMJ) disc, which is a crucial piece of the Puzzle in tissue engineering efforts. Toward this end, cell types were identified and quantified regionally in the TMJ disc.Materials and Methods: Porcine TMJ discs were examined by histology, electron microscopy, and immunohistochemistry. Histology consisted of hematoxylin and eosin staining to identify regional variation of cell type and cell numbers. Transmission electron microscopy was used to elucidate differences in organelle content and pericellular matrix between TMJ disc cells and chondrocytes from hyaline cartilage. Immunohistochemistry was used to assess the presence of smooth and skeletal muscle character in the TMJ disc.Results: The overall ratio of fibroblasts to chondrocyte-like cells in the TMJ disc was approximately 2.35 to 1, with the highest relative number of chondrocyte-like cells in the intermediate zone. Electron microscopy revealed distinct differences between TMJ disc chondrocyte-like cells and chondrocytes from hyaline cartilage with respect to organelles and the pericellular region. Immunostaining identified smooth muscle in the form of vessels, which were most prominent in the anterior band. Skeletal muscle was not observed.Conclusion: The cells of the TMJ disc are distinctly different from cells of hyaline cartilage, and consequently should not be referred to as chondrocytes. TMJ disc cells are comprised of heterogeneously distributed subpopulations, with fibroblasts predominating over fibrochondrocytes. (c) 2006 American Association of Oral and Maxillofacial Surgeons.