Acute Rabbit Eye Model for Testing Subretinal Prostheses.
Acute Rabbit Eye Model for Testing Subretinal Prostheses.
复制标题
用于测试视网膜下假体的急性兔眼模型。
DOI：
10.1167/tvst.8.5.20
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发表时间：
2019
影响因子：
3
通讯作者：
Cheng,Lingyun
中科院分区：
文献类型：
--
作者：
Xiao,Ying;Wang,Yuqin;Li,Fangting;Lin,Tiezhu;Huffman,Kristyn;Landeros,Stephanie;Bosse,Brandon;Jing,Yi;Bartsch,Dirk-Uwe;Thorogood,Scott;Freeman,WilliamR;Cheng,Lingyun
目的：视网膜下假体是一种新的技术，用于恢复视网膜色素变性或年龄相关性黄斑变性患者的有用视力。我们的特点是手术植入技术和功能的时间窗口的急性兔眼模型，用于测试人类subretinal prosthes.Methods：视网膜假体植入subretinally在26只兔子使用两步技术。术后第1 ~ 21天行眼底成像、荧光素眼底血管造影和光学相干断层扫描（OCT）检查，观察假体位置和视网膜解剖结构的变化。结果：84.6%的兔眼植入成功，视网膜贴壁良好。OCT显示植入后前2天覆盖的视网膜为全层。直到第3天，组织学证实了覆盖视网膜的完整内层。反复OCT显示上覆视网膜进行性萎缩;术后第5天和第6天约有40%的视网膜厚度保留。结论：两步植入技术适用于兔眼模型与人类假体。兔视网膜可用于急性电生理测试的视网膜假体，但不适合慢性研究，由于merangiotic视网膜和其有限的时间窗口validation.Translational Relevance：在假体手术中使用这种技术的改善疗效将规避挑战的动物模型，提供类似人类的功能，关键的过渡到人类的临床试验。
Purpose: Subretinal prostheses are a novel technology for restoring useful vision in patients with retinitis pigmentosa or age-related macular degeneration. We characterize the surgical implantation technique and functional time window of an acute rabbit eye model for testing of human subretinal prostheses.Methods: Retinal prostheses were implanted subretinally in 26 rabbits using a two-step technique. Fundus imaging, fluorescein fundus angiography, and optical coherence topography (OCT) were conducted postoperatively from days 1 to 21 to monitor prosthesis positioning and retinal anatomic changes.Results: Successful implantation and excellent retina apposition were achieved in 84.6% of the rabbits. OCTs showed the overlying retina at full thickness for the first 2 days after implantation. Histology confirmed intact inner layers of the overlying retina until day 3. Progressive atrophy of the overlying retina was revealed by repeated OCTs; approximately 40% of the retina thickness remained on postoperative days 5 and 6.Conclusions: The two-step implantation technique works well for the rabbit eye model with human prostheses. Rabbit retina may be used for acute electrophysiologic testing of a retinal prosthesis, but is unsuitable for chronic studies due to the merangiotic retina and its limited time window of validity.Translational Relevance: The improved efficacy in prosthesis surgery using this technique will circumvent the challenges in animal models that provide human-like features critical for the transition into human clinical trials.