Cytokinin treatment delays senescence but not sucrose loss in harvested broccoli

Cytokinin treatment delays senescence but not sucrose loss in harvested broccoli
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DOI:
10.1016/s0925-5214(97)01419-1
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发表时间:
1997-06-01
影响因子:
7
通讯作者:
Davey, MC
Davey, MC
中科院分区:
农林科学1区
文献类型:
--
作者:
Downs, CG;Somerfield, SD;Davey, MC

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我们详细描述了收获的西兰花花组织(Brassica oleracea L.)视觉退化之前和伴随的生理变化,并表明其中一些变化在细胞分裂素(6-苄基氨基嘌呤)处理后发生了改变。细胞分裂素处理并不能防止收获后小花中蔗糖的快速流失。在收获后的前6小时,对照和处理组织中的蔗糖浓度都下降了约50%。然而,在细胞分裂素处理后,天冬酰胺和谷氨酰胺浓度的大幅增加延迟了48小时以上。细胞分裂素处理还改变了通常伴随西兰花小花变黄的生理变化。细胞分裂素处理后,氨基酸和可溶性蛋白的下降和后期氨浓度的升高均有所延迟。我们讨论了这些结果与细胞分裂素处理对调节采后衰老的可能机制的影响。(C) 1997爱思唯尔科学有限公司
We describe details of the physiological changes that both precede and accompany visual deterioration in harvested broccoli floral tissues (Brassica oleracea L.), and show that some of these changes are altered after treatment with the cytokinin, 6-benzylaminopurine. Cytokinin treatment did not prevent the rapid loss of sucrose that occurs in florets after harvest. During the first 6 h after harvest, sucrose concentration declined by approximately 50% in both controls and treated tissues. However, after cytokinin treatment the large increases in asparagine and glutamine concentration were delayed by more than 48 h. Cytokinin treatment also altered the physiological changes which usually accompany the yellowing of broccoli florets. The decline in amino acids and soluble proteins, and the late increase in ammonia concentration were all delayed after cytokinin treatment. We discuss these results in relation to the effects of cytokinin treatment on the possible mechanisms regulating postharvest senescence. (C) 1997 Elsevier Science B.V.