Anesthesia-induced neurodegeneration in fetal rat brains.
Anesthesia-induced neurodegeneration in fetal rat brains.
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DOI：
10.1203/pdr.0b013e3181b3381b
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发表时间：
2009-10
影响因子：
3.6
通讯作者：
Wei H
中科院分区：
文献类型：
--
作者：
Wang S;Peretich K;Zhao Y;Liang G;Meng Q;Wei H
我们研究了异氟醚在宫内暴露的孕鼠胚胎上诱导神经变性的程度。孕21天的大鼠分为三个实验组。对照组大鼠自主呼吸100%氧气1小时。给药组大鼠通过气管内导管吸入1.3%或3%异氟烷（含100%氧气）并进行机械通气1小时。给药后6小时通过剖腹产分娩大鼠幼仔，从每个胎仔心脏的左心室采集胎仔血液，并评价S100β。然后使用半胱天冬酶-3免疫组织化学法在海马CA 1区和压后皮质（RS）中评价胎脑的细胞凋亡。与对照组和1.3%异氟烷组相比，3%异氟烷治疗组显示出显著更高水平的S100β水平和显著增加的CA 1海马和RS皮质中总caspase-3阳性细胞的平均密度。对照组和1.3%异氟醚组之间的S100β水平或caspase-3阳性细胞密度无差异。异氟醚浓度为3%，持续1小时，增加了发育中的胎鼠大脑海马CA 1区和压后皮质的神经变性。
We investigated the extent of isoflurane induced neurodegeneration on the fetuses of pregnant rats exposed in utero. Pregnant rats at gestational day 21 were divided into three experimental groups. Rats in the control group spontaneously breathed 100% oxygen for one hour. Rats in the treatment groups breathed either 1.3% or 3% isoflurane in 100% oxygen through an endotracheal tube with mechanical ventilation for one hour. Rat pups were delivered by Caesarian section six hours after treatment and fetal blood was sampled from the left ventricle of each fetal heart and evaluated for S100β. Fetal brains were then evaluated for apoptosis using caspase-3 immunohistochemistry in the CA1 region of the hippocampus and the retrosplenial cortex (RS). The 3% isoflurane treatment group showed significantly higher levels of S100β levels and significantly increased average densities of total caspase-3 positive cells in the CA1 hippocampus and RS cortex as compared to the control and 1.3% isoflurane groups. There were no differences in S100β levels or densities of caspase-3 positive cells between the control and 1.3% isoflurane groups. Isoflurane at a concentration of 3% for one hour increased neurodegeneration in the hippocampal CA1 area and the retrosplenial cortex in the developing brain of fetal rats.