Simultaneous Electrophysiology and Optogenetic Perturbation of the Same Neurons in Chronically Implanted Animals using μLED Silicon Probes.
Simultaneous Electrophysiology and Optogenetic Perturbation of the Same Neurons in Chronically Implanted Animals using μLED Silicon Probes.
复制标题
使用 μLED 硅探针对长期植入动物中的相同神经元进行同步电生理学和光遗传学扰动。
DOI：
10.1101/2023.02.05.527184
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Diba,Kamran
中科院分区：
文献类型：
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作者：
Kinsky,NathanielR;Vöröslakos,Mihály;Ruiz,JoseRobertoLopez;WatkinsdeJong,Laurel;Slager,Nathan;McKenzie,Sam;Yoon,Euisik;Diba,Kamran
微型发光二极管（μLED）硅探针的特点是在多柄探针的每个柄中的多个位置嵌入独立可控的微型发光二极管（LED），从而实现时间和空间上精确的光遗传神经回路询问。在这里，我们提出了一个协议进行因果关系和可重复的神经回路操纵慢性植入，自由移动的动物。我们描述了引入光遗传学构建体、制备和植入μLED探针、使用局灶性光遗传学扰动进行体内电生理学检查以及在实验终止后回收探针的步骤。有关本方案使用和执行的完整详细信息，请参见Watkins de Jong et al.（2023年）.1
Micro-light-emitting-diode (μLED) silicon probes feature independently controllable miniature light-emitting-diodes (LEDs) embedded at several positions in each shank of a multi-shank probe, enabling temporally and spatially precise optogenetic neural circuit interrogation. Here, we present a protocol for performing causal and reproducible neural circuit manipulations in chronically implanted, freely moving animals. We describe steps for introducing optogenetic constructs, preparing and implanting a μLED probe, performing simultaneousin vivoelectrophysiology with focal optogenetic perturbation, and recovering a probe following termination of an experiment.For complete details on the use and execution of this protocol, please refer to Watkins de Jong et al. (2023).1