Aberrant intracellular calcium signaling in olfactory neurons from patients with bipolar disorder
Aberrant intracellular calcium signaling in olfactory neurons from patients with bipolar disorder
复制标题
DOI：
10.1176/appi.ajp.162.3.616
复制
发表时间：
2005-03-01
影响因子：
17.7
通讯作者：
Rawson, NE
中科院分区：
文献类型：
--
作者：
Hahn, CG;Gomez, G;Rawson, NE
目的：作者研究了使用活体患者的嗅觉受体神经元来测试双相情感障碍患者神经元细胞群中钙信号是否改变的可行性。方法：使用比率荧光显微镜来评估来自7名无药物治疗的双相情感障碍患者的活检来源的嗅觉受体神经元中细胞内钙水平的基础和刺激诱导的变化，10个情绪稳定剂治疗的双相情感障碍患者，和17个年龄和性别匹配的比较科目没有bipolar disorder.Results：嗅觉受体神经元从7个无药物治疗的患者主要反应刺激与细胞内钙离子的减少，不像那些从7个匹配的健康受试者。用情绪稳定剂治疗的患者的嗅觉受体神经元不太可能比无药物patients.Conclusions的嗅觉受体神经元对刺激作出反应：这项研究表明了使用嗅觉受体神经元来检查活体患者神经元细胞内信号转导改变的可行性。尽管我们的结果基于少数受试者，但表明嗅觉受体神经元细胞内钙信号的改变可能是双相情感障碍的一个特征。
Objective: The authors examined the feasibility of using olfactory receptor neurons from living patients to test whether calcium signaling is altered in a neuronal cell population in bipolar disorder.Method: Ratiometric fluorescence photomicroscopy was used to assess basal and stimulus-induced changes in intracellular calcium levels in biopsy-derived olfactory receptor neurons from seven euthymic patients with bipolar disorder who were medication-free, 10 euthymic patients with bipolar disorder who were treated with mood stabilizers, and 17 age- and sex-matched comparison subjects without bipolar disorder.Results: Olfactory receptor neurons from the seven medication-free patients responded to stimuli predominantly with decreases in intracellular calcium, unlike those from the seven matched healthy subjects. Olfactory receptor neurons from patients treated with mood stabilizers were less likely to respond to stimulation than olfactory receptor neurons from medication-free patients.Conclusions: This study demonstrates the feasibility of using olfactory receptor neurons to examine alterations in intracellular signaling in neuronal cells from living patients. Our results, although based on a small number of subjects, suggest that altered intracellular calcium signaling in olfactory receptor neurons may be a trait of bipolar disorder.