Characterization of a 2-Cys peroxiredoxin IV in Marsupenaeus japonicus (kuruma shrimp) and its role in the anti-viral immunity
Characterization of a 2-Cys peroxiredoxin IV in Marsupenaeus japonicus (kuruma shrimp) and its role in the anti-viral immunity
复制标题
日本对虾 (Marsupenaeus japonicus) 2-Cys 过氧化还原蛋白 IV 的表征及其在抗病毒免疫中的作用
DOI：
10.1016/j.fsi.2013.09.018
复制
发表时间：
2013-12-01
影响因子：
4.7
通讯作者：
Kang, Cui-Jie
中科院分区：
文献类型：
--
作者：
Chen, Xiao-Wei;Kang, Li-Hua;Kang, Cui-Jie
越来越多的证据表明，过氧化物还毒素（Prx）是多种传染病发病机制中的关键分子，是癌症等重大疾病的潜在治疗靶点。在本研究中，我们报道了日本有麻猴（Marsupenaeus japonicus）中一个具有过氧化物酶活性并参与抗白斑综合征病毒（WSSV）免疫应答的过氧化物酶（Prx IV），命名为MjPrx IV。MjPrx IV是一种245个氨基酸的多肽，具有预测的19个氨基酸的信号肽，Ahpc-TSA结构域和1-Cys PrxC结构域。系统发育分析显示该蛋白属于Pix IV亚家族。MjPrx IV转录本在虾的鳃、肝胰腺、心脏、胃、卵巢、精子和肠组织中检测到，并且在WSSV攻击后在性腺、鳃和红细胞中表达上调。成熟的MjPrx IV肽在大肠杆菌系统中重组表达。该蛋白具有过氧化物酶活性。此外，dsRNA抑制MjPrx IV增加了虾体内WSSV的复制，而rMjPrx IV注射到虾体内则减少了WSSV的复制。这些数据提示MjPrx IV在对虾抗病毒免疫中具有重要作用。据我们所知，这项研究是首次报道虾Prx IV具有抗wssv活性。(C) 2013 Elsevier Ltd.版权所有。
Accumulating evidence suggests that peroxiredoxins (Prx) are key molecules in the pathogenesis of various infectious diseases and are potential therapeutic targets for major diseases such as cancers. In this study, we report a peroxiredoxin IV (Prx IV) in Marsupenaeus japonicus, designated as MjPrx IV, which exhibited peroxidase activity and participated in the anti-white spot syndrome virus (WSSV) immune response. MjPrx IV is a 245-amino acid polypeptide with a predicted 19-amino acid signal peptide, an Ahpc-TSA domain, and a 1-Cys PrxC domain. Phylogenetic analysis revealed that the protein belongs to the Pix IV subfamily. MjPrx IV transcripts were detected in the gills, hepatopancreas, heart, stomach, ovaries, spermary, and intestine tissues, and are upregulated in the gonads, gills and hemocytes of shrimp after WSSV challenge. The mature MjPrx IV peptide was recombinantly expressed in an Escherichia coli system. The protein exhibited peroxidase activity. Furthermore, dsRNA suppression of MjPrx IV increased WSSV replication in shrimp, whereas rMjPrx IV injection into shrimp decreased WSSV replication. These data suggest that MjPrx IV has an important role in shrimp antiviral immunity. To our knowledge, this study is the first to report a shrimp Prx IV that has anti-WSSV activity. (C) 2013 Elsevier Ltd. All rights reserved.