Molecular mapping in tropical maize (Zea mays L.) using microsatellite markers. 2. Quantitative trait loci (QTL) for grain yield, plant height, ear height and grain moisture.
Molecular mapping in tropical maize (Zea mays L.) using microsatellite markers. 2. Quantitative trait loci (QTL) for grain yield, plant height, ear height and grain moisture.
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DOI：
10.1111/j.1601-5223.2003.01667.x
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发表时间：
2004-01
期刊：
影响因子：
2.7
通讯作者：
S. T. Sibov;Cláudio Lopes de Souza;A. Garcia;Adelmo Rezende Silva;A. Garcia;C. A. Mangolin;Luciana Lasry Benchimol;A. P. de Souza
中科院分区：
文献类型：
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作者：
S. T. Sibov;Cláudio Lopes de Souza;A. Garcia;Adelmo Rezende Silva;A. Garcia;C. A. Mangolin;Luciana Lasry Benchimol;A. P. de Souza
利用已有的包含117个微卫星位点和400个F(2)植株的遗传图谱，对热带玉米进行了数量性状位点定位。对自交F(2)植株获得的400个F（2:3）系进行了QTL鉴定，并在5个环境中进行了2次重复评价。用这5种环境的平均值进行QTL测定。测定了籽粒产量（GY）、株高（PH）、穗高（EH）和水分（GM）。基因型(G)、环境(E)和GxE互作对所有性状的方差均极显著。遗传率为0.69，PH为0.66，EH为0.67，GM为0.23。利用复合区间作图（CIM），共鉴定出13个不同的qtl: GY为4个，PH为4个，EH为5个。未检测到GM的QTL。QTL解释了GY、PH和EH表型变异的32.73%、24.76%和20.91%。13个qtl主要表现为部分显性或过显性基因作用，定位于染色体1、2、7、8和9。这些性状的高值QTL等位基因大多来自L-14-4B系。定位分析发现，与两个或多个性状相关的基因组区域与性状之间的相关性一致，支持QTL之间的多效性或紧密连锁。发现的qtl数量较少，可能是由于热带环境中存在很大的差异。
A previous genetic map containing 117 microsatellite loci and 400 F(2) plants was used for quantitative trait loci (QTL) mapping in tropical maize. QTL were characterized in a population of 400 F(2:3) lines, derived from selfing the F(2) plants, and were evaluated with two replications in five environments. QTL determinations were made from the mean of these five environments. Grain yield (GY), plant height (PH), ear height (EH) and grain moisture (GM) were measured. Variance components for genotypes (G), environments (E) and GxE interaction were highly significant for all traits. Heritability was 0.69 for GY, 0.66 for PH, 0.67 for EH and 0.23 for GM. Using composite interval mapping (CIM), a total of 13 distinct QTLs were identified: four for GY, four for PH and five for EH. No QTL was detected for GM. The QTL explained 32.73 % of the phenotypic variance of GY, 24.76 % of PH and 20.91 % of EH. The 13 QTLs displayed mostly partial dominance or overdominance gene action and mapped to chromosomes 1, 2, 7, 8 and 9. Most QTL alleles conferring high values for the traits came from line L-14-4B. Mapping analysis identified genomic regions associated with two or more traits in a manner that was consistent with correlation among traits, supporting either pleiotropy or tight linkage among QTL. The low number of QTLs found, can be due to the great variation that exists among tropical environments.