Identification of a movement protein of rice yellow stunt rhabdovirus
Identification of a movement protein of rice yellow stunt rhabdovirus
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DOI：
10.1128/jvi.79.4.2108-2114.2005
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发表时间：
2005-02-01
影响因子：
5.4
通讯作者：
Fang, RX
中科院分区：
文献类型：
--
作者：
Huang, YW;Geng, YF;Fang, RX
水稻黄矮弹状病毒（Rice yellow stunt rhabdovirus，RYSV）在其负义RNA基因组中编码7个基因，顺序为3 '-N-P-3-M-G-6-L-5'。RYSV基因组中基因3和位于P和M基因之间的其它植物弹状病毒的类似基因的存在构成了植物弹状病毒的独特特征，其不同于其中P和M基因直接连锁的动物感染弹状病毒。然而，很少有人知道这些额外的植物弹状病毒基因的功能。在这里，我们提供的证据表明，由基因3编码的蛋白质产品的RYSV，P3，具有几个属性相关的病毒细胞到细胞的运动蛋白（NIP）。蛋白质一级和二级结构分析表明，RYSV P3是病毒MP的“30 K”超家族的成员。基因枪反式互补实验表明，RYSV P3可以支持移动缺陷型马铃薯X病毒突变体在烟草叶片中的细胞间移动。此外，Northwestern印迹分析表明，RYSV P3蛋白可以在体外结合单链RNA，这是病毒MP的共同特征。最后，谷胱甘肽S-转移酶下拉试验揭示了RYSV P3蛋白和N蛋白之间的特定相互作用，N蛋白是核糖核衣壳的主要成分，核糖核衣壳是一种亚病毒结构，被认为参与植物弹状病毒的细胞间运动。总之，这些数据表明RYSV P3很可能是RYSV的NIP，因此代表了植物弹状病毒特征化MP的第一个例子。
Rice yellow stunt rhabdovirus (RYSV) encodes seven genes in its negative-sense RNA genome in the order 3'-N-P-3-M-G-6-L-5'. The existence of gene 3 in the RYSV genome and an analogous gene(s) of other plant rhabdoviruses positioned between the P and M genes constitutes a unique feature for plant rhabdoviruses that is distinct from animal-infecting rhabdoviruses in which the P and M genes are directly linked. However, little is known about the function of these extra plant rhabdovirus genes. Here we provide evidence showing that the protein product encoded by gene 3 of RYSV, P3, possesses several properties related to a viral cell-to-cell movement protein (NIP). Analyses of the primary and secondary protein structures suggested that RYSV P3 is a member of the "30K" superfamily of viral MPs. Biolistic bombardment transcomplementation experiments demonstrated that RYSV P3 can support the intercellular movement of a movement-deficient potexvirus mutant in Nicotiana benthamiana leaves. In addition, Northwestern blot analysis indicated that the RYSV P3 protein can bind single-stranded RNA in vitro, a common feature of viral MPs. Finally, glutathione S-transferase pull-down assays revealed a specific interaction between the RYSV P3 protein and the N protein which is a main component of the ribonucleocapsid, a subviral structure believed to be involved in the intercellular movement of plant rhabdoviruses. Together, these data suggest that RYSV P3 is likely a NIP of RYSV, thus representing the first example of characterized MPs for plant rhabdoviruses.