Specific egg yolk antibodies (IgY) confer protection against Acinetobacter baumannii in a murine pneumonia model
Specific egg yolk antibodies (IgY) confer protection against Acinetobacter baumannii in a murine pneumonia model
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DOI：
10.1111/jam.14135
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发表时间：
2019-02-01
影响因子：
4
通讯作者：
Astaneh, S. Darvish Alipour
中科院分区：
文献类型：
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作者：
Jahangiri, A.;Owlia, P.;Astaneh, S. Darvish Alipour
目的鲍曼不动杆菌（Acinetobacter baumannii）被认为是死亡率最高的六大危险微生物之一，对人类健康的威胁日益严重。然而，由于缺乏有效的抗生素或商业疫苗，治疗其感染是困难的。通过注射特异性抗体（如IgY）进行被动免疫，可能是一种有吸引力的实用解决方案。方法与结果采用酶联免疫吸附法（ELISA）检测鲍曼不动杆菌外膜重组蛋白OmpA和Omp34的抗原性及灭活全细胞的抗原性。此外，在小鼠肺炎模型中评估了针对这些抗原的特异性IgY抗体（禽抗体）的预防作用。特异性IgY抗体在肺炎模型中具有多种预防作用。在触发抗体和提供保护方面，OmpA是最有效的抗原。虽然在ELISA中观察到针对OmpA和Omp34组合的抗体（称为Omp33-36和Omp34 kDa）产生的协同效应，但在挑战中意外地发现了拮抗效应。造成这种现象的原因还有待明确。结论所有IgY特异性抗体对鲍曼不动杆菌引起的小鼠肺炎均有保护作用。本研究的意义和影响特异性IgY抗体可作为鲍曼不动杆菌引起肺炎的药物。
Aim Acinetobacter baumannii, an increasingly serious health threat, is considered as one of the six most dangerous microbes of high mortality rate. However, treatment of its infections is difficult because of the lack of efficient antibiotic or commercial vaccines. Passive immunization through administration of specific antibodies such as IgY, could be an attractive practical solution. Methods and Results In the current study, antigenicity of two recombinant outer membrane proteins (OmpA and Omp34) as well as inactivated whole cell of A. baumannii was assessed by ELISA. Moreover, prophylactic effects of specific IgY antibodies (avian antibody) raised against these antigens were evaluated in a murine pneumonia model. The specific IgY antibodies had various prophylactic effects in the pneumonia model. OmpA was the most potent antigen in terms of triggering antibody and conferring protection. While a synergic effect was observed in ELISA for antibodies raised against a combination of OmpA and Omp34 (which are known as Omp33-36 and Omp34 kDa), an antagonistic effect was unexpectedly seen in challenges. The reason for this phenomenon remains to be precisely addressed. Conclusion All the specific IgY antibodies could protect mice against pneumonia caused by A. baumannii. Significance and Impact of the Study The specific IgY antibodies could be employed as a pharmaceutical against pneumonia caused by A. baumannii.