Molecular mapping of the Ph-3 gene for late blight resistance in tomato
Molecular mapping of the Ph-3 gene for late blight resistance in tomato
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DOI：
10.1080/14620316.2002.11511493
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发表时间：
2002-05-01
影响因子：
1.9
通讯作者：
Hanson, P
中科院分区：
文献类型：
--
作者：
Chunwongse, J;Chunwongse, C;Hanson, P
晚疫病由致病疫霉 (Phytophthora infestans (Mont.) deBary) 引起，是凉爽、潮湿环境下番茄 (Lycopersicon esculentum Mill.) 的主要病害。在这项研究中，我们报告了 L. pimpinellifolium (Jusi.) Mill 基因的定位。登记号 L3708 调节对致病疫霉的种族特异性抗性。对来自CLN657（易感）X L3708（抗性）和晚疫病抗性分离的杂交的F-2番茄植物进行了对克服宿主抗性基因Ph和Ph-2的致病疫霉分离株Pi-16的抗性的评估。抗性和易感 DNA 库，通过从九个抗性 F2 植物（R 库）和八个易感 F2 植物（S 库）中大量收集等量 DNA 来创建，并且 DNA 库用于筛选扩增片段长度多态性 (AFLP) 引物。使用 120 个三碱基选择性核苷酸引物对组合的聚合酶链式反应 (PCR) 显示了来自抗性亲本和 R 库特有的 5 个引物对的 5 个 DNA 带，以及来自与易感亲本和 S 库相关的一对引物对的 DNA 带。 DNA条带分离和克隆后，将两个与抗性相关的单拷贝DNA克隆与源自L. esculentum X L. pennellii的50个基因渗入系（IL）杂交； IL9-3 含有来自 9 号染色体长臂的基因渗入，是唯一的多态性 IL。使用映射到 9 号染色体的限制性片段长度多态性 (RFLP) 标记和两个 AFLP 衍生的 RFLP 标记来探测第二个 F2 群体。在 RFLP 标记 TG591 处发现一个 LOD 得分为 18.41 的主要数量性状位点（QTL）；它占方差的 71.4%。该基因 Ph-3 与番茄晚疫病抗性基因 Ph（之前映射到 7 号染色体或 Ph-2（映射到 10 号染色体））非等位基因。
Late blight, caused by Phytophthora infestans (Mont.) deBary, is a major disease of tomato (Lycopersicon esculentum Mill.) in cool, wet environments. In this study we report on the mapping of a gene in L. pimpinellifolium (Jusi.) Mill. accession L3708 conditioning race-specific resistance to P. infestans. F-2 tomato plants from the cross CLN657 (susceptible) X L3708 (resistant) and segregating for late blight resistance were evaluated for resistance to P. infestans isolate Pi-16 that overcomes host resistance genes Ph and Ph-2. Resistant and susceptible DNA pools, created by bulking equal amounts of DNA from nine resistant F2 plants (for R-pool) and eight susceptible F2 plants (for S-pool), and the DNA pools were used to screen amplified fragment length polymorphic (AFLP) primers. Polymerase chain reaction (PCR) using 120 combinations of three-base selective nucleotide primer pairs revealed five DNA bands from five primer pairs unique to the resistant parent and R-pool, and a DNA band from one primer pair associated with the susceptible parent and S-pool. After DNA band isolation and cloning, two single-copied DNA clones associated with resistance were hybridized with 50 introgression lines (IL) derived from L. esculentum X L. pennellii; IL9-3, containing an introgression from the long arm of chromosome 9, was the only polymorphic IL. Restriction fragment length polymorphism (RFLP) markers mapped to chromosome 9 and two AFLP-derived RFLP markers were used to probe a second F2 population. A major quantitative trait locus (QTL) with an LOD score of 18.41 was found at RFLP marker TG591; it accounted for 71.4% of the variance. This gene, Ph-3, is non-allelic to tomato late blight resistance genes Ph (previously mapped to chromosome 7 or Ph-2 (mapped to chromosome 10)).