denV gene of bacteriophage T4 restores DNA excision repair to mei-9 and mus201 mutants of Drosophila melanogaster.
denV gene of bacteriophage T4 restores DNA excision repair to mei-9 and mus201 mutants of Drosophila melanogaster.
复制标题
噬菌体 T4 的 denV 基因恢复了黑腹果蝇 mei-9 和 mus201 突变体的 DNA 切除修复。
DOI：
10.1073/pnas.86.9.3227
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发表时间：
1989
影响因子：
11.1
通讯作者：
deRiel,JK
中科院分区：
文献类型：
--
作者：
Banga,SS;Boyd,JB;Valerie,K;Harris,PV;Kurz,EM;deRiel,JK
将T4噬菌体的denV基因融合到果蝇hsp70 （70-kDa热休克蛋白）启动子中，通过p -element介导的转化将其导入果蝇种系。该基因的蛋白产物（内切酶V）用酶学和免疫印迹法在热休克转化子的提取物中检测。该蛋白恢复了该生物体对mei-9和mus201突变体的切除修复和紫外线抗性。这些结果表明，denV基因可以补偿两种非常不同的真核突变体中的切除修复缺陷，因为mus201突变体是其他生物体中典型的切除缺陷突变体，而mei-9突变体表现出广泛的多效性，包括强烈的减数分裂缺陷。本研究允许将DNA修复的分子分析扩展到高等真核生物的种系。这也为未来研究其他具有良好特征的微生物修复基因对这种后生动物生殖系DNA损伤的影响提供了一个模型系统。
The denV gene of bacteriophage T4 was fused to a Drosophila hsp70 (70-kDa heat shock protein) promoter and introduced into the germ line of Drosophila by P-element-mediated transformation. The protein product of that gene (endonuclease V) was detected in extracts of heat-shocked transformants with both enzymological and immunoblotting procedures. That protein restores both excision repair and UV resistance to mei-9 and mus201 mutants of this organism. These results reveal that the denV gene can compensate for excision-repair defects in two very different eukaryotic mutants, in that the mus201 mutants are typical of excision-deficient mutants in other organisms, whereas the mei-9 mutants exhibit a broad pleiotropism that includes a strong meiotic deficiency. This study permits an extension of the molecular analysis of DNA repair to the germ line of higher eukaryotes. It also provides a model system for future investigations of other well-characterized microbial repair genes on DNA damage in the germ line of this metazoan organism.