A smartphone-controlled amperometric immunosensor for the detection of Pacific ciguatoxins in fish
A smartphone-controlled amperometric immunosensor for the detection of Pacific ciguatoxins in fish
复制标题
DOI：
10.1016/j.foodchem.2021.131687
复制
发表时间：
2021-12-08
期刊：
影响因子：
8.8
通讯作者：
Diogene, Jorge
中科院分区：
文献类型：
--
作者：
Campas, Monica;Leonardo, Sandra;Diogene, Jorge
雪卡毒素(CTX)是由冈比亚属和福岛属微藻产生的海洋神经毒素。CTX可能通过食物网到达人类，并导致雪卡特拉鱼中毒(CFP)。利用多壁碳纳米管(MWCNT)修饰的碳电极和智能手机控制的恒电位仪，研制了一种检测鱼类中太平洋CTX的免疫传感器。该传感器对鱼肉中CTX1B的检出限和定量限分别为6和27pg/mL，分别为0.001和0.005微克/千克。在对来自日本和斐济的鱼类样品的分析中，发现夹心酶联免疫吸附分析(ELISA)、基于细胞的分析(CBA)和LC-MS/MS联用(LC-MS/MS)具有良好的相关性。预测了在零下20摄氏度下至少3个月的稳定性。该生物传感器在2小时多一点的时间内就能提供可靠、准确和精确的太平洋鱼提取物中环磷酰胺含量，适用于监测和研究项目。
Ciguatoxins (CTXs) are marine neurotoxins produced by microalgae of the genera Gambierdiscus and Fukuyoa. CTXs may reach humans through food webs and cause ciguatera fish poisoning (CFP). An immunosensor for the detection of Pacific CTXs in fish was developed using multiwalled carbon nanotube (MWCNT)-modified carbon electrodes and a smartphone-controlled potentiostat. The biosensor attained a limit of detection (LOD) and a limit of quantification (LOQ) of 6 and 27 pg/mL of CTX1B, respectively, which were 0.001 and 0.005 mu g/kg in fish flesh. In the analysis of fish samples from Japan and Fiji, excellent correlations were found with sandwich enzyme-linked immunosorbent assays (ELISAs), a cell-based assay (CBA) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Stability of at least 3 months at-20 degrees C was predicted. In just over 2 h, the biosensor provides reliable, accurate and precise Pacific CTX contents in fish extracts, being suitable for monitoring and research programs.