Lipid Peroxidation by the Manganese Peroxidase of Phanerochaete chrysosporium Is the Basis for Phenanthrene Oxidation by the Intact Fungus
Lipid Peroxidation by the Manganese Peroxidase of Phanerochaete chrysosporium Is the Basis for Phenanthrene Oxidation by the Intact Fungus
复制标题
DOI：
10.1128/aem.60.6.1956-1961.1994
复制
发表时间：
1994-06
影响因子：
4.4
通讯作者：
M. Moen;K. Hammel
中科院分区：
文献类型：
--
作者：
M. Moen;K. Hammel
锰过氧化物酶（MNP）的黄孢原毛平革菌支持Mn（II）依赖，H2 O2独立的脂质过氧化，如所示的两个结果：亚麻酸过氧化，得到的产品与硫代巴比妥酸反应，和亚油酸过氧化，得到己醛。MnP还支持菲缓慢氧化为2，2 ′-联苯甲酸，该反应需要Mn（II），氧气和不饱和脂质。菲氧化为联苯二甲酸的完整培养的P. chrysosporium发生在体外氧化的程度相同，并刺激锰。这些结果支持的作用，锰过氧化物酶介导的脂质过氧化作用的菲氧化P. chrysosporium。
The manganese peroxidase (MnP) of Phanerochaete chrysosporium supported Mn(II)-dependent, H2O2-independent lipid peroxidation, as shown by two findings: linolenic acid was peroxidized to give products that reacted with thiobarbituric acid, and linoleic acid was peroxidized to give hexanal. MnP also supported the slow oxidation of phenanthrene to 2,2′-diphenic acid in a reaction that required Mn(II), oxygen, and unsaturated lipids. Phenanthrene oxidation to diphenic acid by intact cultures of P. chrysosporium occurred to the same extent that oxidation in vitro did and was stimulated by Mn. These results support a role for MnP-mediated lipid peroxidation in phenanthrene oxidation by P. chrysosporium.