Transferrin receptor is a marker of malignant phenotype in human pancreatic cancer and in neuroendocrine carcinoma of the pancreas
Transferrin receptor is a marker of malignant phenotype in human pancreatic cancer and in neuroendocrine carcinoma of the pancreas
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DOI：
10.1016/j.ejca.2004.01.036
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发表时间：
2004-06-01
影响因子：
8.4
通讯作者：
Schmidt, J
中科院分区：
文献类型：
--
作者：
Ryschich, E;Huszty, G;Schmidt, J
转铁蛋白受体（TFRC）是一种膜结合蛋白，在增殖细胞（如恶性细胞）中的表达量大于在静止细胞中的表达量。在实体肿瘤中，TFRC的特异性表达可以作为一种诊断工具或治疗靶点。TFRC是否在人类胰腺肿瘤中表达尚不清楚。本研究的目的是研究TFRC和转铁蛋白在人胰腺癌和胰腺神经内分泌肿瘤中的表达。手术后获得51例人胰腺癌标本和14例胰腺神经内分泌肿瘤标本。采用标准免疫组化方法研究TFRC、转铁蛋白和细胞角蛋白的表达。采用流式细胞术研究TFRC在9种导管性胰腺癌细胞系中的体外表达情况。与正常组织相比，93%的胰腺肿瘤细胞显示TFRC阳性（82%）或异质（11%）表达。在恶性上皮细胞中强烈表达；正常间质细胞和内皮细胞未被抗tfrc抗体染色。原发肿瘤和转移瘤的TFRC表达频率相似。3例神经内分泌癌肿瘤细胞TFRC阳性表达。TFRC在胰腺良性神经内分泌肿瘤中表达为阴性。胰腺癌细胞系在体外具有TFRC低表达的特点。因此，与正常胰腺组织和良性胰腺神经内分泌肿瘤相比，胰腺癌和神经内分泌癌通常以TFRC高表达为特征。因此，TFRC是胰腺恶性转化的标志，可作为潜在的诊断和治疗靶点。(C) 2004 Elsevier Ltd.版权所有。
Transferrin receptor (TFRC) is a membrane-bound protein expressed in larger amounts in proliferating, e.g., malignant, cells than in quiescent cells. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Whether TFRC is expressed in human pancreatic tumours is unknown. The aim of this study was the investigation of the expression of TFRC and transferrin in human pancreatic cancer and in neuroendocrine tumours of the pancreas. Fifty one specimens of human pancreatic cancer and 14 samples of pancreatic neuroendocrine tumours were obtained after surgery. The expression of TFRC, transferrin and cytokeratin was studied by standard immunohistochemistry. Flow cytometry was used for the investigation of TFRC expression in nine cell lines of ductal pancreatic cancer in vitro. In contrast to normal tissue, 93% of pancreatic tumour cells showed positive (82%) or heterogeneous (11%) expression of TFRC. It was strongly expressed by malignant epithelial cells; normal stromal and endothelial cells were not stained by anti-TFRC antibodies. Primary tumours and metastases showed a similar frequency of TFRC expression. Three neuroendocrine carcinomas showed positive expression of TFRC by malignant tumour cells. The expression of TFRC was negative in benign neuroendocrine tumours of the pancreas. The cell lines of pancreatic cancer were characterised by a low expression of TFRC in vitro. In contrast to normal pancreatic tissue and benign neuroendocrine tumours of the pancreas, pancreatic cancer and neuroendocrine carcinoma are therefore characterised frequently by high expression of TFRC. Hence, TFRC represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target. (C) 2004 Elsevier Ltd. All rights reserved.