Comparative Transcriptomic and Phenotypic Analysis of the Responses of Bacillus cereus to Various Disinfectant Treatments
Comparative Transcriptomic and Phenotypic Analysis of the Responses of Bacillus cereus to Various Disinfectant Treatments
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DOI：
10.1128/aem.03003-09
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发表时间：
2010-05-01
影响因子：
4.4
通讯作者：
Abee, Tjakko
中科院分区：
文献类型：
--
作者：
Ceragioli, Mara;Mols, Maarten;Abee, Tjakko
抗菌化学品广泛应用于清洁和消毒食品接触表面。然而，细菌对各种消毒剂的细胞反应尚不清楚。在这项研究中，蜡状芽孢杆菌ATCC 14579暴露于四种不同的消毒剂（苯扎氯铵，次氯酸钠，过氧化氢和过氧乙酸）的生理和全基因组的转录反应进行了分析。对于每种消毒剂，确定导致生长衰减、生长停滞和细胞死亡的浓度。转录组分析表明，B.蜡状芽孢杆菌在暴露于选定浓度的消毒剂后，诱导了共同和特异性反应。值得注意的是，共同的反应包括参与一般和氧化应激反应的基因。暴露于苯扎氯铵（一种已知可诱导膜损伤的消毒剂），可特异性诱导参与脂肪酸代谢的基因。苯扎氯铵诱导的膜损伤，证实了荧光显微镜，脂肪酸分析显示调制的脂肪酸组成的细胞膜。暴露于次氯酸钠诱导参与硫和含硫氨基酸代谢的基因，这与次氯酸钠应激细胞中观察到的巯基过度氧化有关。暴露于过氧化氢和过氧乙酸诱导高度相似的反应，包括参与DNA损伤修复和SOS反应的基因的上调。值得注意的是，过氧化氢和过氧乙酸处理的细胞表现出高突变率与诱导SOS反应。
Antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. However, the cellular response of bacteria to various disinfectants is unclear. In this study, the physiological and genome-wide transcriptional responses of Bacillus cereus ATCC 14579 exposed to four different disinfectants (benzalkonium chloride, sodium hypochlorite, hydrogen peroxide, and peracetic acid) were analyzed. For each disinfectant, concentrations leading to the attenuation of growth, growth arrest, and cell death were determined. The transcriptome analysis revealed that B. cereus, upon exposure to the selected concentrations of disinfectants, induced common and specific responses. Notably, the common response included genes involved in the general and oxidative stress responses. Exposure to benzalkonium chloride, a disinfectant known to induce membrane damage, specifically induced genes involved in fatty acid metabolism. Membrane damage induced by benzalkonium chloride was confirmed by fluorescence microscopy, and fatty acid analysis revealed modulation of the fatty acid composition of the cell membrane. Exposure to sodium hypochlorite induced genes involved in metabolism of sulfur and sulfur-containing amino acids, which correlated with the excessive oxidation of sulfhydryl groups observed in sodium hypochlorite-stressed cells. Exposures to hydrogen peroxide and peracetic acid induced highly similar responses, including the upregulation of genes involved in DNA damage repair and SOS response. Notably, hydrogen peroxide- and peracetic acid-treated cells exhibited high mutation rates correlating with the induced SOS response.