Development of a Mouse Model of Supraspinatus Tendon Insertion Site Healing
Development of a Mouse Model of Supraspinatus Tendon Insertion Site Healing
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DOI：
10.1002/jor.22727
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发表时间：
2015-01-01
影响因子：
2.8
通讯作者：
Andarawis-Puri, Nelly
中科院分区：
文献类型：
--
作者：
Bell, Rebecca;Taub, Peter;Andarawis-Puri, Nelly
冈上肌（SS）肌腱撕裂是常见的肌肉骨骼损伤，其手术修复表现出任何肌腱再撕裂的最高发生率。由于缺乏一种允许生物扰动识别无效愈合机制的模型，改善SS肌腱愈合的治疗方法的发展受到损害。本研究的目的是通过创建可复制的SS肌腱脱离和手术修复来建立冈上肌插入部位愈合的小鼠模型，该模型可应用于各种近交系小鼠和基因突变株。解剖和结构分析证实，小鼠的肩袖与人类相似，包括存在coraco肩峰（CA）弓和显示纤维软骨过渡区的插入部位。在骨科研究中常用的7种小鼠身上成功进行了手术修复，这表明该手术可以应用于大多数近交系和基因突变体。术后14天，两种小鼠品系的组织学证实了修复的质量，这代表了所评估小鼠品系的差异。开发的小鼠模型将使我们能够研究涉及插入部位愈合的机制。(c) 2014年骨科研究会。Wiley期刊公司出版。[J]中华骨科杂志，2015。
Supraspinatus (SS) tendon tears are common musculoskeletal injuries whose surgical repair exhibits the highest incidence of re-tear of any tendon. Development of therapeutics for improving SS tendon healing is impaired by the lack of a model that allows biological perturbations to identify mechanisms that underlie ineffective healing. The objective of this study was to develop a mouse model of supraspinatus insertion site healing by creating a reproducible SS tendon detachment and surgical repair which can be applied to a wide array of inbred mouse strains and genetic mutants. Anatomical and structural analyses confirmed that the rotator cuff of the mouse is similar to that of human, including the presence of a coracoacromial (CA) arch and an insertion site that exhibits a fibrocartilagenous transition zone. The surgical repair was successfully conducted on seven strains of mice that are commonly used in Orthopaedic Research suggesting that the procedure can be applied to most inbred strains and genetic mutants. The quality of the repair was confirmed with histology through 14 days after surgery in two mouse strains that represent the variation in mouse strains evaluated. The developed mouse model will allow us to investigate mechanisms involved in insertion site healing. (c) 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:25-32, 2015.