MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10
MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10
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DOI：
10.7150/jca.40438
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发表时间：
2020-01-01
影响因子：
3.9
通讯作者：
Jiang, Qingping
中科院分区：
文献类型：
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作者：
Liu, Wenya;Cai, Tonghui;Jiang, Qingping
鼻咽癌（Nasopharyngeal carcinoma，NPC）是我国南方及东南亚地区最常见的恶性肿瘤之一。MYH 10是NMMHC-IIB蛋白的编码基因。已有研究表明MYH 10在乳腺癌、胶质瘤和脑膜瘤中表达上调。此外，它被miR 200家族靶向。但在NPC中尚未发现相关研究。在48例鼻咽癌组织中，MYH 10在癌组织中的表达明显低于癌旁正常组织。MYH 10的缺失可促进NPC的迁移和侵袭。此外，我们证明了在miR-200家族中，miR-200 a对MYH 10的调节作用最强。miR-200 a模拟物可降低MYH 10的表达，而miR-200 a抑制剂可增加MYH 10的表达。接下来，我们使用双荧光素酶报告基因发现miR-200 a直接与MYH 10结合。最后，证明siMYH 10可以逆转miR-200 a抑制剂对NPC细胞迁移和侵袭的影响。综上所述，可以得出结论：MYH 10在鼻咽癌中的表达较癌旁组织低，MYH 10的缺失可促进鼻咽癌细胞的迁移和侵袭;在miR-200家族中，miR-200 a对MYH 10的调控作用最强; MYH 10是miR 200 a的直接靶基因，miR 200 a靶向MYH 10调节NPC细胞的迁移和侵袭。
Nasopharyngeal carcinoma (NPC), is one of the most common malignant tumor in southern China and southeast Asia. MYH10 is a coding gene of the NMMHC-IIB protein. Previous studies have shown that MYH10 expression was up-regulated in breast cancer, glioma and meningioma. Moreover, it was targeted by miR200 family. However, no relevant studies have been found in NPC. In present study, we found in 48 NPC specimens, MYH10 level was lower in most cancer areas than that in the adjacent normal tissue. Moreover, the depletion of MYH10 can promote the migration and invasion of NPC. In addition, we demonstrated that miR-200a has the strongest regulation to MYH10 among miR-200 family. miR 200a mimics could decrease MYH10 expression, while miR-200a inhibitor increase MYH10 expression. Next, we found that miR-200a bound directly to MYH10 using Dual-luciferase reporter. Finally, it was demonstrated that siMYH10 could reverse the effect of miR-200a inhibitor on NPC cell migration and invasion. Taken together, it can be concluded that MYH10 is lowly expressed in NPC compared with adjacent tissues, and the loss of MYH10 can promote the migration and invasion of NPC cells; Among the miR-200 family, miR-200a has the strongest regulatory effect on MYH10; MYH10 is a direct target gene of miR200a, and miR200a targets MYH10 to regulate the migration and invasion of NPC cells.