Media conditioned by coculture of pericytes and endothelial cells under a hypoxic state stimulate in vitro angiogenesis.
Media conditioned by coculture of pericytes and endothelial cells under a hypoxic state stimulate in vitro angiogenesis.
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通过在缺氧状态下共培养周细胞和内皮细胞而调节的培养基刺激体外血管生成。
DOI：
10.1159/000267370
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发表时间：
1994
影响因子：
2.1
通讯作者：
K. Sueishi
中科院分区：
文献类型：
--
作者：
T. Murata;T. Ishibashi;H. Inomata;K. Sueishi
我们研究了周细胞在视网膜新生血管形成中的作用以及缺氧对其的影响。条件培养基（CM）分别从培养的周细胞、视网膜毛细血管内皮细胞（RCEs）或共培养的周细胞和RCEs中获取，O2浓度分别为1、5和20%。然后研究了这些CM对胶原凝胶中rce的毛细血管样管形成的影响。低氧环境下（1和5% O2）周细胞和周细胞与rce共培养的CM显著促进了rce的管状形成（p < 0.01），而20% O2共培养的CM则有抑制作用（p < 0.01）。本研究表明，周细胞在缺氧条件下，即在1和5% O2下释放血管生成因子，而在20% O2下与rce共培养时释放血管抑制因子。
We investigated the role of pericytes in retinal neovascularization and the effect of hypoxia on it. Conditioned media (CM) were harvested from cultured pericytes, retinal capillary endothelial cells (RCEs) or cocultured pericytes and RCEs at 1, 5 and 20% O2. The effect of these CM on the capillary-like tube formation of RCEs in the collagen gel was then investigated. The CM harvested from either pericytes or coculture of pericytes and RCEs in a hypoxic environment (1 and 5% O2) significantly enhanced the tube formation of RCEs (p < 0.01), while the CM of coculture at 20% O2 showed an inhibitory effect (p < 0.01). The present study suggests that pericytes release angiogenic factors under hypoxic conditions, i.e. at 1 and 5% O2, while they release angiostatic factors when they are cocultured with RCEs at 20% O2.