Imaging intracellular viscosity of a single cell during photoinduced cell death
Imaging intracellular viscosity of a single cell during photoinduced cell death
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DOI：
10.1038/nchem.120
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发表时间：
2009-04-01
期刊：
影响因子：
21.8
通讯作者：
Ogilby, Peter R.
中科院分区：
文献类型：
--
作者：
Kuimova, Marina K.;Botchway, Stanley W.;Ogilby, Peter R.
扩散介导的细胞过程，如代谢，信号传导和运输，取决于细胞内基质的流体动力学性质。用于治疗癌症的光动力疗法依赖于在药物照射时细胞内产生短寿命的细胞毒性剂。这种治疗的功效取决于细胞毒性剂必须扩散通过的介质的粘度。在这里，光谱分辨荧光测量卟啉二聚体为基础的分子转子被用来量化单细胞的细胞内粘度的变化。我们发现，有一个显着增加的粘度的直接环境中的转子光诱导细胞死亡。这种粘度增加的效果直接观察到的光敏化的形成和衰减的一个关键的细胞毒性剂，单线态分子氧的扩散依赖动力学。使用这些工具，我们提供了深入了解细胞中的扩散动力学，这是有关药物输送，细胞信号和细胞内质量运输。
Diffusion-mediated cellular processes, such as metabolism, signalling and transport, depend on the hydrodynamic properties of the intracellular matrix. Photodynamic therapy, used in the treatment of cancer, relies on the generation of short-lived cytotoxic agents within a cell on irradiation of a drug. The efficacy of this treatment depends on the viscosity of the medium through which the cytotoxic agent must diffuse. Here, spectrally resolved fluorescence measurements of a porphyrin-dimer-based molecular rotor are used to quantify intracellular viscosity changes in single cells. We show that there is a dramatic increase in the viscosity of the immediate environment of the rotor on photoinduced cell death. The effect of this viscosity increase is observed directly in the diffusion-dependent kinetics of the photosensitized formation and decay of a key cytotoxic agent, singlet molecular oxygen. Using these tools, we provide insight into the dynamics of diffusion in cells, which is pertinent to drug delivery, cell signalling and intracellular mass transport.