Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling
Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling
复制标题
使用同量异位标签进行相对和绝对定量蛋白质组学分析，评估华勒变性期间大鼠脊髓神经中的不同蛋白质表达模式。
DOI：
10.4103/1673-5374.265556
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发表时间：
2020-02-01
影响因子：
6.1
通讯作者：
Wang, Yu
中科院分区：
文献类型：
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作者：
Wei, Shuai;Liang, Xue-Zhen;Wang, Yu
周围神经的感觉纤维和运动纤维具有不同的解剖结构和损伤后的再生功能。为了清楚地了解这些差异背后的生物学过程，我们使用了一种称为同量异位素标签的相对和绝对定量的标记技术来研究Sprague-Dawley大鼠脊神经组织的蛋白质谱。在对沃勒变性的反应中，在感觉神经中总共筛选了626种蛋白质，其中368种上调，258种下调。此外，在运动神经中筛选出637个蛋白，其中372个上调，265个下调。使用基因本体论和京都基因百科全书和生物信息学的基因组分析来分析所有鉴定的蛋白质，并且使用定量实时聚合酶链反应分析来测试和验证与沃勒变性密切相关的几个关键蛋白质的存在。仅在感觉神经中鉴定的差异表达蛋白主要与包括细胞-细胞粘附、碳水化合物代谢过程和细胞粘附的各种生物过程相关，而仅在运动神经中鉴定的差异表达蛋白主要与糖酵解过程、细胞氧化还原稳态和蛋白质折叠相关的生物过程相关。在细胞成分方面，感觉神经和运动神经中差异表达的蛋白质通常与细胞外exosomes、髓鞘和粘着斑有关。根据京都基因和基因组百科全书，鉴定的差异表达蛋白主要与各种类型的代谢途径有关。总之，本研究筛选差异表达的蛋白质，以揭示更多关于感觉和运动神经之间的差异和相似性在沃勒变性。本研究结果可为进一步研究Wallerian变性中感觉神经和运动神经的差异以及周围神经再生的特点提供参考。
Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the differences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between sensory and motor nerves in Wallerian degeneration and the characteristics of peripheral nerve regeneration.