Relationship between the expression of TNFR1-RIP1/RIP3 in peripheral blood and cognitive function in occupational Al-exposed workers: A mediation effect study
Relationship between the expression of TNFR1-RIP1/RIP3 in peripheral blood and cognitive function in occupational Al-exposed workers: A mediation effect study
复制标题
职业铝接触工人外周血TNFR1-RIP1/RIP3表达与认知功能关系的中介效应研究
DOI：
10.1016/j.chemosphere.2021.130484
复制
发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Niu Qiao
中科院分区：
文献类型：
--
作者：
Zhang Jingsi;Hao Yanxia;Wang Yanni;Han Yingchao;Zhang Shuhui;Niu Qiao
铝（Al），不是生物活性所必需的，在组织中积累。它对神经系统产生毒性作用，导致人类不可逆转的认知障碍。本研究采用整群抽样方法，观察某大型铝厂长期职业性铝接触工人的认知功能，并测定其外周血肿瘤坏死因子受体1（TNFR 1）、受体相互作用蛋白1（RIP 1）和RIP 3的表达。在血液巨噬细胞和小胶质细胞中表达的TNF-α及其受体TNFR 1、TR 1和TR 3增强神经元的坏死性凋亡。此外，探讨TNFR 1，RIP 1和RIP 3在长期职业铝接触工人外周血中的表达与其认知功能变化的关系。3组间画钟测验（CDT）得分分布差异有统计学意义（P< 0.05）。相关分析结果显示RIP 1和RIP 3蛋白含量与简易精神状态检查量表（MMSE）和CDT评分呈负相关（P< 0.05）。血浆铝含量与其他生物学指标呈正相关（P< 0.05），与MMSE、CDT评分呈负相关（P< 0.05）。结果表明，RIP 3蛋白在血浆铝含量与认知功能之间具有不完全的中介作用。这表明Al可能通过影响神经系统中TNFR 1、RIP 1和RIP 3的表达来影响认知功能。
Aluminium (Al), not essential for biological activities, accumulates in the tissues. It exerts toxic effects on the nervous system, inducing in humans’ irreversible cognitive impairment. In this study, a cluster sampling method was used to observe the cognitive function of long-term occupational Al-exposed workers in a large Al factory, and determine the expression of peripheral blood tumour necrosis factor receptor 1 (TNFR1), receptor-interacting protein 1 (RIP1), and RIP3. TNF-alpha, expressed in blood macrophages and microglia, with its receptors TNFR1, TR1 and TR3, enhances the necroptosis of neurons. Additionally, the relationship between the expression of TNFR1, RIP1, and RIP3 in the peripheral blood of long-term occupational Al-exposed workers and changes in their cognitive function was explored. The differences in the distributions of clock drawing test (CDT) scores among the three groups were statistically significant (P< 0.05). The results of correlation analysis showed that RIP1 and RIP3 protein contents were negatively correlated with mini-mental state examination (MMSE) and CDT scores (P< 0.05). Plasma Al content was positively correlated with other biological indicators (P< 0.05), and negatively correlated with MMSE and CDT scores (P< 0.05). Results showed that RIP3 protein had an incomplete mediation effect between plasma Al content and cognitive function. This suggests that Al may affect cognitive function by influencing the expression of TNFR1, RIP1, and RIP3 in the nervous system.