Circulating interleukin-18 and osteopontin are useful to evaluate disease activity in patients with tuberculosis
Circulating interleukin-18 and osteopontin are useful to evaluate disease activity in patients with tuberculosis
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DOI：
10.1016/j.cyto.2005.01.014
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发表时间：
2005-05-21
期刊：
影响因子：
3.8
通讯作者：
Uede, T
中科院分区：
文献类型：
--
作者：
Inomata, S;Shijubo, N;Uede, T
背景：辅助性T型1 （Th1）反应参与了结核病的保护性免疫、病理生理和发展。然而，目前尚不清楚哪些分子反映了结核病患者的疾病活动。方法：采用特异性酶免疫法测定循环干扰素。测定47例肺结核患者和7例军人结核患者抗结核治疗前的ifn - γ、白细胞介素-12 （IL-12）、IL-18和骨桥蛋白（OPN），并测定19例结核患者抗结核治疗前后的ifn - γ、IL-18和OPN。结果：肺结核患者循环ifn - γ、IL-18和OPN水平明显高于正常对照组，而循环IL-12水平与正常对照组无显著差异。循环ifn - γ、IL-12、IL-18和OPN与肺部病变程度呈平行关系，循环ifn - γ、IL-18和OPN与肺结核患者发热程度呈平行关系。军人结核患者的循环OPN、ifn - γ和IL-18水平极高。抗结核治疗显著降低循环IL-18和OPN，而循环IL-12和ifn - γ则无显著降低。结论：在Th1应答相关分子中，IL-18和OPN的循环水平，而不是ifn - γ或IL-12，反映了结核病患者的疾病活动性。(c) 2005 Elsevier Ltd版权所有。
Background: T helper type 1 (Th1) responses have been implicated in the protective immunity, pathophysiology and development of tuberculosis. However, it is still unclear which molecule(s) reflect disease activity in patients with tuberculosis.Methods: By specific enzyme immunoassays, circulating interferon-gamma. (IFN-gamma), interleukin-12 (IL-12), IL-18 and osteopontin (OPN) were measured in 47 patients with pulmonary tuberculosis and 7 patients with miliary tuberculosis before anti-tuberculosis therapy, and also measured in 19 patients with tuberculosis before and after anti-tuberculosis therapy.Results: Circulating IFN-gamma, IL-18 and OPN levels were significantly higher in patients with pulmonary tuberculosis than in healthy controls, while there was no significant difference in levels of circulating IL-12 between tuberculosis patients and controls. Circulating IFN-gamma, IL-12, IL-18 and OPN paralleled the extent of lung lesions, and circulating IFN-gamma, IL-18 and OPN paralleled the magnitude of fever in patients with pulmonary tuberculosis. Patients with miliary tuberculosis had extremely high levels of circulating OPN, IFN-gamma and IL-18. Circulating IL-18 and OPN were significantly decreased with anti-tuberculosis therapy, whereas circulating IL-12 and IFN-gamma were not.Conclusions: Among Th1 response associated molecules, circulating levels of IL-18 and OPN, but not IFN-gamma or IL-12, reflect disease activity in patients with tuberculosis.(c) 2005 Elsevier Ltd. All rights reserved.