Gut-derived intraepithelial lymphocytes induce long term immunity against Toxoplasma gondii.
Gut-derived intraepithelial lymphocytes induce long term immunity against Toxoplasma gondii.
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DOI：
10.4049/jimmunol.161.9.4902
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发表时间：
1998-11
影响因子：
4.4
通讯作者：
A. Lepage;D. Buzoni–Gatel;D. Bout;L. Kasper
中科院分区：
文献类型：
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作者：
A. Lepage;D. Buzoni–Gatel;D. Bout;L. Kasper
肠道上皮内淋巴细胞(IEL)是预防口腔获得性病原体感染的重要屏障。通过将抗原启动的IEL转移到幼稚宿主中可以保护其免受攻击。使用小鼠模型，我们在两个基因不同的小鼠品系(C57BL/6和CBA/J)上证明了在口腔感染含有缓殖子的包囊后的特定时间可以分离出保护性IEL。在这些特定的时间段从感染小鼠的肠道过继转移IEL可以提供长期的保护，这是由死亡率和针对攻击的包囊数量确定的。保护性IEL似乎是CD8+、TCR-α/β，至少部分依赖于宿主中TCR-γ/Delta T细胞的存在。内源性产生的关键细胞因子，干扰素-γ，对宿主免疫是必不可少的。这些发现表明，肠道来源的IEL是一种潜在的重要机制，可以为宿主提供长期免疫。
Intraepithelial lymphocytes (IEL) of the intestine represent an important barrier in the prevention of infection against orally acquired pathogens. Adoptive transfer of Ag-primed IEL into a naive host can protect against challenge. Using a murine model, we demonstrate in two genetically distinct mouse strains (C57BL/6 and CBA/J) that protective IEL can be isolated at specific times after oral infection with cysts containing bradyzoites. Adoptive transfer of IEL obtained from the intestine of infected mice at these specific times can provide long term protection, as determined by mortality and cyst number against challenge. The protective IEL appear to be CD8+, TCR-alpha/beta and are at least partially dependent upon the presence of TCR-gamma/delta T cells in the host. Endogenous production of the pivotal cytokine, IFN-gamma, is essential for host immunity. These findings demonstrate that gut-derived IEL represent a potentially important mechanism to provide long term immunity to the host.