Telomere shortening and DNA damage of embryonic stem cells induced by cigarette smoke
Telomere shortening and DNA damage of embryonic stem cells induced by cigarette smoke
复制标题
香烟烟雾引起胚胎干细胞端粒缩短和DNA损伤
DOI：
10.1016/j.reprotox.2012.07.003
复制
发表时间：
2013-01-01
影响因子：
3.3
通讯作者：
Liu, Lin
中科院分区：
文献类型：
--
作者：
Huang, Junjiu;Okuka, Maja;Liu, Lin
胚胎干细胞（ESCs）提供了一个有价值的体外模型，用于测试化学品和环境污染物（包括香烟烟雾）的毒性。小鼠胚胎干细胞急性或慢性暴露于烟雾成分，香烟烟雾冷凝物（CSC），或镉，丰富的CSC的组成部分，然后评估其自我更新，凋亡，DNA损伤和端粒功能。高剂量CSC或镉对ESCs的急性暴露可增加DNA损伤和凋亡。然而，ESCs表现出显着的能力，恢复后的曝光。长期暴露于低剂量CSC或镉导致ESCs端粒缩短和DNA损伤。总之，ESC急性暴露于CSC或镉会导致细胞立即死亡并降低多能性，而ESC慢性暴露于CSC或镉会导致DNA损伤和端粒缩短。值得注意的是，在传代过程中选择一部分ESC以抵抗烟雾诱导的端粒氧化损伤。(C)2012 Elsevier Inc. All rights reserved.
Embryonic stem cells (ESCs) provide a valuable in vitro model for testing toxicity of chemicals and environmental contaminants including cigarette smoke. Mouse ESCs were acutely or chronically exposed to smoke components, cigarette smoke condensate (CSC), or cadmium, an abundant component of CSC, and then evaluated for their self-renewal, apoptosis, DNA damage and telomere function. Acute exposure of ESCs to high dose of CSC or cadmium increased DNA damage and apoptosis. Yet, ESCs exhibited a remarkable capacity to recover following absence of exposure. Chronic exposure of ESCs to low dose of CSC or cadmium resulted in shorter telomeres and DNA damage. Together, acute exposure of ESCs to CSC or cadmium causes immediate cell death and reduces pluripotency, while chronic exposure of ESCs to CSC or cadmium leads to DNA damage and telomere shortening. Notably, a sub-proportion of ESCs during passages is selected to resist to smoke-induced oxidative damage to telomeres. (C) 2012 Elsevier Inc. All rights reserved.