Role of genes that modulate host immune responses in the immunogenicity and pathogenicity of vaccinia virus.
Role of genes that modulate host immune responses in the immunogenicity and pathogenicity of vaccinia virus.
复制标题
调节宿主免疫反应的基因在痘苗病毒的免疫原性和致病性中的作用。
DOI：
10.1128/jvi.79.10.6554-6559.2005
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发表时间：
2005
影响因子：
5.4
通讯作者：
Panicali,DennisL
中科院分区：
文献类型：
--
作者：
Jackson,ShawnS;Ilyinskii,Petr;Philippon,Valérie;Gritz,Linda;Yafal,AliciaGómez;Zinnack,Kimberly;Beaudry,KristinR;Manson,KelledyH;Lifton,MichelleA;Kuroda,MarceloJ;Letvin,NormanL;Mazzara,GailP;Panicali,DennisL
Poxvirus vaccine vectors, although capable of eliciting potent immune responses, pose serious health risks in immunosuppressed individuals. We therefore constructed five novel recombinant vaccinia virus vectors which contained overlapping deletions of coding regions for the B5R, B8R, B12R, B13R, B14R, B16R, B18R, and B19R immunomodulatory gene products and assessed them for both immunogenicity and pathogenicity. All five of these novel vectors elicited both cellular and humoral immunity to the inserted HIV-BH10envcomparable to that induced by the parental Wyeth strain vaccinia virus. However, deletion of these immunomodulatory genes did not increase the immunogenicity of these vectors compared with the parental vaccinia virus. Furthermore, four of these vectors were slightly less virulent and one was slightly more virulent than the Wyeth strain virus in neonatal mice. Attenuated poxviruses have potential use as safer alternatives to current replication-competent vaccinia virus. Improved vaccinia virus vectors can be generated by deleting additional genes to achieve a more significant viral attenuation.
