Identification of an essential gene encoding a class-V unconventional myosin in Drosophila melanogaster
Identification of an essential gene encoding a class-V unconventional myosin in Drosophila melanogaster
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DOI：
10.1046/j.1432-1327.1998.2570529.x
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发表时间：
1998-11-01
期刊：
影响因子：
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通讯作者：
Bownes, M
中科院分区：
文献类型：
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作者：
MacIver, B;McCormack, A;Bownes, M
V类肌球蛋白是一类独特的肌球蛋白马达，在细胞内转运中发挥作用。小鼠稀释基因是这类被克隆的第一个成员，突变导致毛色变浅或神经源性缺陷导致过早死亡。V类肌球蛋白的其他实例已在酵母、鸡和大鼠中描述。在这里，我们报告的克隆的第一个类V肌球蛋白从果蝇。我们表明，这种肌球蛋白的表达主要是在成年生殖系和早期胚胎，并在卵子发生过程中，转录本定位于卵母细胞。遗传和原位杂交实验已经确定该基因位于43 C区域。我们有证据表明，它映射到一个突变，在这个地区与胚胎致死表型。
Class-V myosins are a unique type of myosin motor with roles in intracellular transport. The mouse dilute gene was the first member of this class to be cloned, with mutations resulting in lightening of the coat colour or neurogenic defects leading to early death. Further examples of class-V myosins have been described in yeast, chicken and rat. Here, we report the cloning of the first class-V myosin from Drosophila. We show that expression of this myosin is predominantly in the adult germ line and early embryo and that the transcript is localised in the oocyte during oogenesis. Genetic and in situ hybridisation experiments have determined that this gene is located in the 43C region. We have evidence that it maps to a mutation in this region with an embryonic lethal phenotype.