Effect of growth factors on matrix synthesis by ligament fibroblasts
Effect of growth factors on matrix synthesis by ligament fibroblasts
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DOI：
10.1002/jor.1100150104
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发表时间：
1997-01-01
影响因子：
2.8
通讯作者：
Woo, SLY
中科院分区：
文献类型：
--
作者：
Marui, T;Niyibizi, C;Woo, SLY
尽管已有报道称多种生长因子对软组织愈合有调节作用，但生长因子在韧带愈合过程中对蛋白质合成的具体影响尚未得到广泛研究。在本研究中，我们观察了碱性和酸性成纤维细胞生长因子、转化生长因子β1和表皮生长因子对体外培养的内侧副韧带和前交叉韧带成纤维细胞合成胶原和非胶原蛋白的影响。用氚-脯氨酸摄取法测定胶原和非胶原蛋白的合成，用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分析合成的胶原蛋白的类型。我们的数据显示，转化生长因子β1以剂量依赖的方式增加内侧副韧带和前十字韧带成纤维细胞的胶原和非胶原蛋白的合成。经转化生长因子β1处理后，培养的内侧副韧带和前十字韧带成纤维细胞的胶原合成增加约为未处理对照组的1.5倍。尽管前交叉韧带成纤维细胞对转化生长因子β1的反应性与内侧副韧带成纤维细胞相当，但其合成的基质蛋白的量约为内侧副韧带成纤维细胞的一半。增加的主要是I型胶原。用表皮生长因子处理前十字韧带成纤维细胞可增加约25%的胶原合成，但对内侧副韧带成纤维细胞几乎没有影响。碱性或酸性成纤维细胞生长因子都不能促进胶原或非胶原蛋白的合成。这些发现表明，局部应用转化生长因子β1，单独或与表皮生长因子联合使用，可能通过增加韧带重塑和愈合过程中的基质合成来增强韧带的强度。
Although it has been reported that several growth factors modulate soft-tissue healing, the specific effects of growth factors on protein synthesis during ligament healing have not been widely investigated. In this study, we examined the effects of basic and acidic fibroblast growth factors, transforming growth factor beta 1, and epidermal growth factor on collagen and noncollagenous protein synthesis by cultured fibroblasts from medial collateral ligament and anterior cruciate ligament in vitro. Uptake of tritiated proline was used to measure synthesis of collagen and noncollagenous protein, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the type of collagens synthesized. Our data showed that transforming growth factor beta 1 increased both collagen and noncollagenous protein synthesis by medial collateral and anterior cruciate ligament fibroblasts on a dose-dependent basis. Collagen synthesis by cultured fibroblasts from the medial collateral and anterior cruciate ligaments was increased by treatment with transforming growth factor beta 1 by as much as approximately 1.5 times that of untreated controls. Although the response to transforming growth factor beta 1 by anterior cruciate ligament fibroblasts was equal to that by medial collateral ligament fibroblasts, the amounts of matrix proteins synthesized by anterior cruciate ligament fibroblasts were approximately half of that by medial collateral ligament fibroblasts. The increase was mostly in type-I collagen. Treatment of anterior cruciate ligament fibroblasts with epidermal growth factor increased collagen synthesis by approximately 25% but had little effect on medial collateral ligament fibroblasts. Neither basic nor acidic fibroblast growth factor increased either collagen or noncollagenous protein synthesis. These findings suggest that topical application of transforming growth factor beta 1, alone or in combination with epidermal growth factor, may have the potential to strengthen the ligament by increasing matrix synthesis during its remodeling and healing processes.