Lactulose ingestion increases faecal bifidobacterial counts:: A randomised double-blind study in healthy humans
Lactulose ingestion increases faecal bifidobacterial counts:: A randomised double-blind study in healthy humans
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DOI：
10.1038/sj.ejcn.1601829
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发表时间：
2004-03-01
影响因子：
4.7
通讯作者：
Flourié, B
中科院分区：
文献类型：
--
作者：
Bouhnik, Y;Attar, A;Flourié, B
目的：人体摄入高剂量乳果糖可能会增加被认为具有促进健康特性的粪便双歧杆菌和乳酸杆菌。低剂量和耐受性良好的乳果糖的作用尚不清楚。本研究的目的是评估长期低剂量乳果糖管理对粪便双歧杆菌和选定的代谢指标可能参与结肠carcinogenesis.Subjects和方法的影响：在所有的，16名健康志愿者被纳入这个对照，随机，双盲，平行组试验。参与者以5 g b.i.d.的剂量摄入乳果糖或安慰剂（蔗糖）。6周。在基线（d 0）、糖摄入3周（d21）和6周（d42）后定期收集粪便。结果：乳果糖组粪便细菌计数高于蔗糖组（P = 0.03）。乳果糖摄入导致从d 0至d21和d42粪便双歧杆菌计数显著增加（（m+/-s.e.m.）8.25+/-0.53，8.96 +/-0.40和9.54+/-0.28对数菌落形成单位/克湿重（CFU/g）（P = 0.048））。安慰剂摄入未导致任何粪便双歧杆菌计数变化。在整个研究过程中，两组的总厌氧菌、乳酸菌和pH值没有显著变化。无论是粪便胆汁酸，也没有中性固醇修改乳果糖。乳果糖组的过度排气更常见（P = 0.03），但非常轻微。两组之间的腹胀和肠鸣没有差异。结论：每天10 g乳果糖的测量值会增加粪便中的双歧杆菌计数，并且乳果糖符合被视为益生元的标准。
Objective: Faecal bifidobacteria and lactobacilli, perceived as exerting health-promoting properties, may be increased by ingestion of high-dose lactulose in humans. The effects of low and well-tolerated doses of lactulose are not well known. The aim of the study was to assess the effects of prolonged low-dose lactulose administration on faecal bifidobacteria and selected metabolic indexes potentially involved in colonic carcinogenesis.Subjects and methods: In all, 16 healthy volunteers were included in this controlled, randomised, double-blind, parallel group trial. Participants ingested lactulose or placebo (sucrose) at a dose of 5 g b.i.d. for 6 weeks. Stools were regularly collected at baseline (d0), and after 3 (d21) and 6 (d42) weeks of sugar ingestion. Tolerance was evaluated using a daily chart.Results: Faecal bifidobacterial counts were higher in lactulose than in sucrose group (P = 0.03). Lactulose ingestion led to a significant increase in faecal bifidobacteria counts from d0 to d21 and d42 ((m+/-s.e.m.) 8.25+/-0.53, 8.96+/-0.40 and 9.54+/-0.28 log colony-forming units/g wet wt (CFU/g), respectively (P = 0.048)). Placebo ingestion did not lead to any faecal bifidobacterial count change. Total anaerobes, Lactobacillus and pH were not significantly changed throughout the study in the two groups. Neither faecal bile acids nor neutral sterols were modified by lactulose. Excess flatus was more common in the lactulose group (P = 0.03), but was very mild. Bloating and borborygmi did not differ between both the groups.Conclusions: A measure of 10 g lactulose/day increases faecal bifidobacterial counts, and lactulose fulfils the criteria requested to be considered as a prebiotic.