BnaUBP15s positively regulates seed size and seed weight in Brassica napus
BnaUBP15s positively regulates seed size and seed weight in Brassica napus
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DOI：
10.1016/j.ocsci.2023.07.002
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发表时间：
2023-09
期刊：
影响因子：
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通讯作者：
Jianwei Gu;Jianyin Chen;Jie Xia;Dengfeng Hong
中科院分区：
文献类型：
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作者：
Jianwei Gu;Jianyin Chen;Jie Xia;Dengfeng Hong
Brassica napus (B. napus) is a globally significant oilseed crop, making a substantial contribution to both human oil and livestock feed production. Enhancing seed weight is crucial for improving rapeseed yield; however, only a limited number of seed weight-related genes have been functionally validated inB. napusthus far.UBIQUITIN-SPECIFIC PROTEASE 15 (UBP15)belongs to the ubiquitin protease pathway and plays a maternal role in prolonging seed development inArabidopsis. The potential utilization of UBP15 for enhancing seed yield inB. napushas remained unexplored until now. In this study, we identified the orthologs ofUBP15inB. napusand investigated its functionality using the CRISPR-Cas9 system. We generated mutant plants with multiple editing types targetingBnaubp15sand successfully isolated T-DNA-free homozygous mutant lines that exhibited edits across four homologs ofBnaUBP15in T2 generation plants. Our preliminary data demonstrated that mutation ofBnaUBP15ssignificantly reduced seed size, seed weight, and plant height while noticeably increasing the number of primary branches. These findings not only provide crucial evidence for further elucidating the molecular mechanism underlying the regulation of seed weight and size byBnaUBP15sbut also offer promising novel germplasm for enhancing plant architecture.
