Ion channels enable electrical communication in bacterial communities.
Ion channels enable electrical communication in bacterial communities.
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DOI：
10.1038/nature15709
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发表时间：
2015-11-05
期刊：
影响因子：
64.8
通讯作者：
Süel GM
中科院分区：
文献类型：
--
作者：
Prindle A;Liu J;Asally M;Ly S;Garcia-Ojalvo J;Süel GM
细菌离子通道的研究为神经元信号传导的结构基础提供了基本的见解。然而，离子通道在细菌中的天然作用仍然难以捉摸。在这里，我们证明离子通道通过钾波的空间传播在细菌生物膜群落内传导长距离电信号。这些波是由正反馈回路产生的，其中代谢触发因素诱导细胞内钾的释放，进而使邻近细胞去极化。通过生物膜传播，这种去极化波协调生物膜内部和外围细胞之间的代谢状态。钾通道的缺失会消除这种反应。正如数学模型所预测的那样，我们进一步表明，钾通道门控的特定遗传扰动可能会阻碍空间传播。总之，这些结果证明了细菌生物膜中离子通道的功能，并为细胞群落中活跃的长距离电信号传导提供了原核范例。
The study of bacterial ion channels has provided fundamental insights into the structural basis of neuronal signaling. However, the native role of ion channels in bacteria has remained elusive. Here we show that ion channels conduct long-range electrical signals within bacterial biofilm communities through spatially propagating waves of potassium. These waves result from a positive feedback loop, in which a metabolic trigger induces release of intracellular potassium, which in turn depolarizes neighboring cells. Propagating through the biofilm, this wave of depolarization coordinates metabolic states among cells in the interior and periphery of the biofilm. Deletion of the potassium channel abolishes this response. As predicted by a mathematical model, we further show that spatial propagation can be hindered by specific genetic perturbations to potassium channel gating. Together, these results demonstrate a function for ion channels in bacterial biofilms, and provide a prokaryotic paradigm for active, long-range electrical signaling in cellular communities.