Long-term culture of normal human kidney glomerular cells.
Long-term culture of normal human kidney glomerular cells.
复制标题
正常人肾肾小球细胞的长期培养。
DOI：
10.1007/bf02634128
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
D'Ambrosio,SM
中科院分区：
文献类型：
--
作者：
Gibson-D'Ambrosio,RE;D'Ambrosio,SM
lost upon subculturing. However, since GGT is a non-specific _.^ _ _, marker of cell type, the more specific URO Mab markers of proxi-mate tubular and glomerular cell types were investigated. Of the 6 URO Mabs available, only the URO-1, URO-3 and 3, 3 NK 0URO-10 recognize antigens unique to either the glomerular or proximate tubular cell type. The URO-2 recognizes both glomerular and proximate tubular antigens, URO-4 recognizes proximate tubu-lar and Henle's loop cellular antigens, and the URO-5 recognizes antigens present in Henle's loop and distal and collecting tubular cells. Thus due to the unique specificity of the URO-1, 3 and 10 Mabs these were used to provide insights into the origin of the cultured cells. The URO-1 Mab was used to detect the expression of the J143 (a 140 000/120 000/30 000 MW glycoprotein) found only in the basement membrane of the glomerulus (4). The expres-sion of the URO-1 glycoprotein in the basement membrane of K923 cells grown at the different subpassages indicated in Fig. 1 was determined immunohistochemically (Fig. 3). The degree of antiURO-1 antibody binding to the basement membrane of the cells in the early subpassages was varied among the two cell morphologies shown in Fig. 1 A. Cells with the polygonal shapes exhibited strong staining, while the elongated-elliptical cells exhibited weak staining for URO-1 Mab (Fig. 3 A). The tightly packed polygonal cells in the second morphological phase also exhibited intense staining for
