Adiponectin Replacement Therapy Attenuates Myocardial Damage in Leptin-deficient Mice with Viral Myocarditis
Adiponectin Replacement Therapy Attenuates Myocardial Damage in Leptin-deficient Mice with Viral Myocarditis
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DOI：
10.1177/147323000503300208
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发表时间：
2005-03
影响因子：
1.6
通讯作者：
T. Takahashi;S. Saegusa;H. Sumino;T. Nakahashi;K. Iwai;S. Morimoto;T. Kanda
中科院分区：
文献类型：
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作者：
T. Takahashi;S. Saegusa;H. Sumino;T. Nakahashi;K. Iwai;S. Morimoto;T. Kanda
观察脂联素替代治疗对瘦素缺乏（OB）小鼠急性病毒性心肌炎心肌损害的影响。脑心肌炎病毒腹腔注射到OB和野生型（WT）小鼠。OB小鼠的一个亚组没有接受任何干预，另一个亚组接受每日脂联素替代，同时进行病毒接种。测定心脏重量、心脏组织学评分、心肌中浸润或凋亡细胞的数量以及肌细胞中脂联素受体的免疫反应性的差异。与WT小鼠的心肌细胞相比，OB小鼠在病毒接种后第4天和第8天的心肌细胞中脂联素受体1的反应性显著降低; OB小鼠的心脏重量也升高，并出现严重的炎症性心肌损伤。OB小鼠的脂联素替代通过增加心肌细胞脂联素受体1的反应性来抑制严重心肌炎的发展。外源性脂联素可通过与脂联素受体1结合抑制瘦素缺乏状态下病毒性心肌炎的进展。
The effects of adiponectin replacement therapy on myocardial damage were studied in leptin-deficient (OB) mice with acute viral myocarditis. Encephalomyocarditis virus was injected intraperitoneally into OB and wild-type (WT) mice. One subgroup of OB mice received no intervention and another subgroup received daily adiponectin replacement, simultaneously with viral inoculation. Differences in heart weight, cardiac histological score, numbers of infiltrating or apoptotic cells in the myocardium and the immunoreactivity of adiponectin receptors in myocytes were determined. The reactivity of adiponectin receptor 1 in myocytes from OB mice on day 4 and day 8 after viral inoculation was significantly decreased compared with that in myocytes from WT mice; the OB mice also had elevated cardiac weights and severe inflammatory myocardial damage. Adiponectin replacement in OB mice inhibited the development of severe myocarditis by augmenting myocyte adiponectin receptor 1 reactivity. Exogenously administered adiponectin may inhibit the progression of viral myocarditis through binding to the adiponectin receptor 1 in leptin-deficient conditions.