MONOCYTES ARE A MAJOR SITE OF PERSISTENCE OF HUMAN CYTOMEGALOVIRUS IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS
MONOCYTES ARE A MAJOR SITE OF PERSISTENCE OF HUMAN CYTOMEGALOVIRUS IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS
复制标题
DOI：
10.1099/0022-1317-72-9-2059
复制
发表时间：
1991-09-01
影响因子：
3.8
通讯作者：
SINCLAIR, JH
中科院分区：
文献类型：
--
作者：
TAYLORWIEDEMAN, J;SISSONS, JGP;SINCLAIR, JH
我们使用巢式聚合酶链反应（PCR）结合荧光激活细胞分选术来确定健康受试者外周血中人巨细胞病毒（HCMV）潜伏期的位点。 将外周血单核细胞（PBM）分离成T细胞或非T细胞群体和单核细胞，然后通过PCR分析HCMV DNA的存在。 在5/6例血清学阳性受试者中，发现HCMV主要存在于非T细胞群体中。 进一步分析表明，病毒存在于贴壁细胞和CD 14+细胞中。 在9例血清阴性受试者中的3例中，我们可以通过PCR重现性地证明HCMV DNA，我们不认为这是由于污染所致。 在这些血清阴性受试者之一中，HCMV DNA主要存在于PBM细胞的非T细胞部分中。 在其余6例血清阴性受试者中未检出HCMV DNA。 我们的结论是，在正常的无症状血清阳性和一些血清阴性的受试者的PBM细胞，HCMV主要存在于单核细胞部分。 此外，在血清阴性受试者中检测到HCMV序列可能表明HCMV感染比常规血清流行病学所显示的更广泛。
We have used the nested polymerase chain reaction (PCR) combined with fluorescence-activated cell sorting to define sites of latency of human cytomegalovirus (HCMV) in the peripheral blood of healthy subjects. Peripheral blood mononuclear (PBM) cells were separated into T cell or non-T cell populations and monocytes, and were then analysed by PCR for the presence of HCMV DNA. In five of six seropositive subjects, HCMV was found predominantly in the non-T cell population. Further analysis suggested that the virus was present in adherent cells and CD14+ cells. In three of nine seronegative subjects we could demonstrate HCMV DNA, which we do not believe was due to contamination, reproducibly by PCR. In one of these seronegative subjects, HCMV DNA was present predominantly in the non-T cell fraction of PBM cells. No HCMV DNA was detectable in the remaining six seronegative subjects. We conclude that, within the PBM cells of normal asymptomatic seropositive and some seronegative subjects, HCMV is present predominantly in the monocyte fraction. In addition, the detection of HCMV sequences in seronegative subjects may indicate that infection with HCMV is more widespread than conventional seroepidemiology suggests.