Recombinant proteins VP1 and VP3 of hepatitis A virus prime for neutralizing response
Recombinant proteins VP1 and VP3 of hepatitis A virus prime for neutralizing response
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甲型肝炎病毒重组蛋白 VP1 和 VP3 用于中和反应
DOI：
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发表时间：
1990
影响因子：
12.7
通讯作者：
F. Deinhardt
中科院分区：
文献类型：
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作者：
V. Gauss;Zhou Mingquan;K. von der Helm;F. Deinhardt
将甲型肝炎病毒（HAV）衣壳蛋白VP1和VP3的6个重叠基因组区域插入表达载体pBD或pUR中，分别表达β-半乳糖苷酶-HAV融合蛋白。重组蛋白溶解性差，因此在放射免疫测定中很难被人抗HAV血清检测到，但溶解在十二烷基硫酸钠中的融合蛋白在免疫印迹中与人和兔抗HAV阳性血清发生反应。 VP1 和 VP3 重组蛋白的抗血清在免疫印迹中与 HAV 各自的结构蛋白发生反应。两种重组蛋白，一种包含 VP1 N 端的前 120 个氨基酸，另一种包含除前 60 个 N 端氨基酸外的所有 VP1，在兔子中诱导瞬时中和抗体反应。在竞争性放射免疫测定中，针对 VP1 和 VP3 其他区域的抗血清既不能中和病毒感染性，也不能识别天然病毒。然而，当免疫动物受到亚免疫原剂量的 HAV 攻击时，所有动物都会产生稳定的病毒中和抗体。
Six overlapping genomic regions of capsid proteins VP1 and VP3 of hepatitis A virus (HAV) inserted into the expression vectors pBD or pUR respectively expressed β‐galactosidase‐HAV fusion proteins. The recombinant proteins were poorly soluble so they were difficult to detect by human anti‐HAV sera in radioimmunoassay, but the fusion proteins dissolved in sodium dodecyl sulfate reacted with human and rabbit anti‐HAV‐positive sera in immunoblots. Antisera against VP1 and VP3 recombinant proteins reacted with the respective structural proteins of HAV in immunoblots. Two recombinant proteins, one including the first 120 amino acids of the N‐terminus of VP1 and the other containing all of VP1 except for the first 60 N‐terminal amino acids, induced a transient neutralizing antibody response in rabbits. Antisera directed against other regions of VP1 and VP3 neither neutralized viral infectivity nor recognized native virus in a competitive radioimmunoassay. However, when immunized animals were challenged with a subimmunogenic dose of HAV, all animals responded with stable virus‐neutralizing antibodies.
人类甲型肝炎病毒基因组的完整核苷酸序列（分离MBB）。
影响因子：
5
作者：
Paul,AV;Tada,H;vonderHelm,K;Wissel,T;Kiehn,R;Wimmer,E;Deinhardt,F
通讯作者：
Deinhardt,F