Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs:: Rapid, simple, and inexpensive method
Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs:: Rapid, simple, and inexpensive method
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DOI：
10.1128/aac.47.11.3616-3619.2003
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发表时间：
2003-11-01
影响因子：
4.9
通讯作者：
Palomino, JC
中科院分区：
文献类型：
--
作者：
Martin, A;Camacho, M;Palomino, JC
耐多药结核病的出现要求进行新的快速药敏试验。采用瑞祖脲微滴比色法和比例法对150株结核分枝杆菌分离株对乙硫胺、卡那霉素、卷曲霉素、氧氟沙星、对氨基水杨酸等二线药物进行了检测。8天后通过目测读数获得mic。瑞祖林微滴度比色法与比例法的结果具有很好的相关性。瑞祖脲微滴比色法价格低廉、快速、操作简单，在资源匮乏的国家实施该方法是可行的。
The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good correlation between results by the colorimetric resazurin microtiter assay and the proportion method was obtained. The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries.