Analysis of genetic relationships of Northeastern Black Bee (Apis mellifera ssp.) in China.
Analysis of genetic relationships of Northeastern Black Bee (Apis mellifera ssp.) in China.
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发表时间：
2009
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通讯作者：
Peng Wen-jun;Luo Qihua;An Jian-dong;Huang Jiaxing;G. Jun
中科院分区：
文献类型：
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作者：
Peng Wen-jun;Luo Qihua;An Jian-dong;Huang Jiaxing;G. Jun
[目的]分析东北黑蜂（Apis mellifera ssp.）摘要对意大利蜜蜂、意大利蜜蜂和高加索蜜蜂与非洲蜜蜂的亲缘关系进行了分析。这对保护意大利蜜蜂的遗传资源具有重要意义。[方法]对A.m.sssp.对黑龙江省国家级自然保护区内9个乡镇和其它西方蜜蜂的HSP 70基因进行了PCR扩增。PCR产物经限制性内切酶DraI酶切后，根据酶切图谱对PCR产物进行克隆和测序，用NJ法构建系统树。[结果] A. m. ssp线粒体DNA tRNAleu-COⅡ区长度为526 bp，只有一个Q序列。P序列缺失。此外，有5个转变（4 T？C，1A？G）和1个颠换（1A？T）的Q序列。结果表明，DraⅠ酶切片段长度分别为422 bp、64 bp、47 bp和41 bp，酶切位点经DNAMAN验证。A.m. ssp.与蜜蜂的进化谱系C和O相似，而与谱系A和M不同。基于NJ树，A. m. ssp。属于O谱系。[结论]线粒体DNA tRNAleu-COⅡ区序列和DraⅠ酶切图谱表明，A. m. ssp.属于O谱系，与同一类群中的阿纳托利亚蜜蜂和高加索蜜蜂亲缘关系最近，与意大利蜜蜂、犬蜜蜂、果蜜蜂亲缘关系较近，而与意大利蜜蜂和非洲蜜蜂亲缘关系较近。基于NJ树，A. m. ssp。是否为两个地理小种的混合群，还是一个新的蜜蜂亚种，有待于进一步研究。
【Objective】 To analyze taxonomy of Northeastern Black Bee (Apis mellifera ssp.), the study was carried out by analyzing the genetic relationships among Apis mellifera mellifera, Apis mellifera ligustica, Apis mellifera canica, Apis mellifera carpatica, Apis mellifera anatoliaca, Apis mellifera caucasica and African bees. This is usefull for the protection of genetic resouces of Apis mellifera ssp. 【Method】 Mitochondrial DNA (mtDNA) tRNAleu-COⅡ region of the A.m.ssp. from 9 towns of National Nature Reserve in China’s Heilongjiang province and other Apis mellifera were amplified by PCR. Then PCR products were digested with restriction enzymes DraI, based on the restriction patterns, PCR products were cloned and sequenced, and the dendrogram was constructed by Neighbor-Joining (NJ) method. 【Result】 The length of mtDNA tRNAleu-COⅡ region were 526 base-pair (bp) , and only one Q sequence was found in A.m.ssp. but P sequence was absent. Besides, there were 5 transitions(4T?C、1A?G) and 1transversion(1A?T)in Q sequence. Only one DraⅠ restriction pattern was identified, the length of restriction fragments was 422 bp, 64 bp, 47 bp and 41 bp, respectively, and the restriction sites were validated by DNAMAN. The sequences and restriction pattern of A.m.ssp. were similar with the evolutionary lineages C and O of Apis meliffera, but different from lineages A and M. Based on the NJ tree, A.m.ssp. belongs to lineage O. 【Conclusion】 The sequence of mtDNA tRNAleu-COⅡ region and DraⅠ restriction pattern indicate that A.m.ssp. belongs to O lineage, and has closest relationship with A.m.anatoliaca and A.m. caucasica in the same group,closer with A.m.ligustica, A.m.canica, A.m. carpatica than that with A.m.mellifera and African bees. Based on the NJ tree,A.m.ssp. mixed in A.m.anatoliaca and A.m. caucasica, whether it is a mixed groups of the two geographical races, or it is a new honey bee subspecies need to be further investigated in the future.