Hydrodynamically Guided Hierarchical Self-Assembly of Peptide-Protein Bioinks
Hydrodynamically Guided Hierarchical Self-Assembly of Peptide-Protein Bioinks
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DOI：
10.1002/adfm.201703716
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发表时间：
2018-04-18
影响因子：
19
通讯作者：
Mata, Alvaro
中科院分区：
文献类型：
--
作者：
Hedegaard, Clara L.;Collin, Estelle C.;Mata, Alvaro
分子自组装和增材制造的有效整合将为生物打印提供技术飞跃。本文报道了一种基于水动力学引导的肽两亲物（PA）与天然存在的生物分子和蛋白质的共组装以产生具有可调分子组成和结构控制的分级结构的生物织物系统。该系统利用液滴按需喷墨打印来利用界面流体力并引导分子自组装成对齐或无序的纳米纤维、不同几何形状和尺寸的水凝胶结构、表面形貌以及通过分子扩散结合的更高有序的构造。PA被设计为在细胞稀释剂条件下在打印期间与一系列细胞外基质（ECM）蛋白和生物分子（包括纤连蛋白、胶原蛋白、角蛋白、弹性蛋白样蛋白和透明质酸）共组装。使用这些分子的组合，NIH-3 T3和脂肪来源的干细胞被生物打印在复杂的结构中，同时表现出高细胞活力（>88%）。通过将自组装与3D生物打印相结合，该研究引入了一种新型的生物织物平台，能够在可调的细胞周围环境中封装和空间分布多种细胞类型。通过这种方式，这项工作证明了这种方法在产生复杂的生物活性支架方面的潜力，这些支架可用于组织工程、体外模型和药物筛选等应用。
Effective integration of molecular self-assembly and additive manufacturing would provide a technological leap in bioprinting. This article reports on a biofabrication system based on the hydrodynamically guided co-assembly of peptide amphiphiles (PAs) with naturally occurring biomolecules and proteins to generate hierarchical constructs with tuneable molecular composition and structural control. The system takes advantage of droplet-on-demand inkjet printing to exploit interfacial fluid forces and guide molecular self-assembly into aligned or disordered nanofibers, hydrogel structures of different geometries and sizes, surface topographies, and higher-ordered constructs bound by molecular diffusion. PAs are designed to co-assemble during printing in cell diluent conditions with a range of extracellular matrix (ECM) proteins and biomolecules including fibronectin, collagen, keratin, elastin-like proteins, and hyaluronic acid. Using combinations of these molecules, NIH-3T3 and adipose derived stem cells are bioprinted within complex structures while exhibiting high cell viability (>88%). By integrating self-assembly with 3D-bioprinting, the study introduces a novel biofabrication platform capable of encapsulating and spatially distributing multiple cell types within tuneable pericellular environments. In this way, the work demonstrates the potential of the approach to generate complex bioactive scaffolds for applications such as tissue engineering, in vitro models, and drug screening.