CORRELATION OF HIGH-SPEED TENSILE-STRENGTH WITH COLLAGEN CONTENT IN CONTROL AND LATHYRITIC RAT SKIN
CORRELATION OF HIGH-SPEED TENSILE-STRENGTH WITH COLLAGEN CONTENT IN CONTROL AND LATHYRITIC RAT SKIN
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DOI：
10.1006/jsre.1993.1004
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发表时间：
1993-01-01
影响因子：
2.2
通讯作者：
SULLIVAN, WG
中科院分区：
文献类型：
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作者：
DOMBI, GW;HAUT, RC;SULLIVAN, WG
撕裂性皮肤损伤的严重程度取决于所施加的载荷和组织的阻力。在低（静态）负荷率下，皮肤拉伸强度与胶原交联程度之间存在高度相关性，由于胶原与糖胺聚糖基质的相互作用，几乎没有增加强度。我们检查了高（弹道）加载速率的影响，以确定胶原交联程度和胶原-基质相互作用对强度的贡献。使用1.5-6月龄大鼠的背部皮肤进行拉伸破坏实验。在背侧的头侧和尾侧位置平行于和横向于身体轴线切割测试样本。使用对照和lathyrogen喂养的大鼠在30%/sec（低速）和6000%/sec（高速）的标称应变速率下测量拉伸强度。进行生化分析以确定总胶原和交联（不溶性）胶原的量。在低速测试中，胶原蛋白含量和皮肤拉伸强度之间存在显著相关性（r = 0.900），横向和平行于脊柱测量。与不溶性胶原含量的相关程度（r= 0.973）高于与总胶原含量的相关程度（r= 0.901）。与对照组相比，lathyrogen饮食的效果产生拉伸强度的显著（P< 0.001）降低（2至3倍）。在高速和低速组中，横向样品的拉伸强度最大，与胶原含量显著相关（r = 0.858）。在高速实验中，在每个测试年龄，与低速结果相比，皮肤拉伸强度显著（P< 0.001）增加（2至3倍）。由于这种增加发生在相同的组织中，这些数据支持我们的假设，即皮肤的动态强度特性涉及基质与胶原相互作用的影响。
Severity of lacerative skin injury depends on the applied load and the resistance of the tissue. At low (static) rates of loading there is a high degree of correlation between skin tensile strength and the degree of collagen crosslinking, with little added strength due to collagen interactions with the glycosaminoglycan matrix. We examined the effects of high (ballistic) rates of loading in order to determine the contributions to strength made by both the degree of collagen crosslinking and the collagen-matrix interaction. Tensile failure experiments were conducted using the dorsal skin of rats 1.5-6 months of age. Test specimen orientations were cut parallel and transverse to the body axis at cephalad and caudad locations on the dorsum. Tensile strength was measured at nominal strain rates of 30%/sec (low speed) and 6000%/sec (high speed) using both control and lathyrogen fed rats. Biochemical analyses were conducted to determine the amount of total and crosslinked (insoluble) collagen. In low-speed tests, there was a significant correlation (r⩾ 0.900) between collagen content and skin tensile strength measured both transverse and parallel to the spine. The degree of correlation was higher with insoluble (r= 0.973) collagen content than with total (r= 0.901) collagen. The effect of a lathyrogen diet produced a significant (P< 0.001) reduction (two- to threefold) in tensile strength compared to control. In both high- and low-speed groups, tensile strength was greatest in the transverse samples with a significant correlation to collagen content (r⩾ 0.858). In the high-speed experiments, the skin tensile strength was significantly (P< 0.001) increased (two- to threefold) compared to low-speed results at each age tested. Because this increase occurred in identical tissues, these data support our hypothesis that the more dynamic strength properties of skin involve the influence of matrix interactions with collagen.