Organization of interphase microtubules in fission yeast analyzed by electron tomography
Organization of interphase microtubules in fission yeast analyzed by electron tomography
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DOI：
10.1016/j.devcel.2007.01.020
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发表时间：
2007-03-01
影响因子：
11.8
通讯作者：
Antony, Claude
中科院分区：
文献类型：
--
作者：
Hoeoeg, Johanna L.;Schwartz, Cindi;Antony, Claude
极化细胞，如神经元细胞、上皮细胞和真菌细胞，都显示出它们的微管（MTs）的特殊组织。杆状分裂酵母（Schizosaccharomyces pombe）的间期MT细胞骨架已被荧光显微镜广泛描述。在这里，我们描述了S. pombe的大规模电子断层扫描研究，包括一个完整真核细胞体积的3D重建，以足够的分辨率显示束中有多少mt及其详细的结构。大多数细胞质mt一端是开放的，另一端是封闭的，这提供了它们极性的证据。在mt本身之间和mt与核膜之间经常观察到电子密集的桥。最后，我们研究了线粒体和囊泡之间的结构/功能关系。我们的分析表明，保存完好的细胞的电子断层扫描非常适合描述精细的超微结构细节，这些细节是以前的技术无法看到的。
Polarized cells, such as neuronal, epithelial, and fungal cells, all display a specialized organization of their microtubules (MTs). The interphase MT cytoskeleton of the rod-shaped fission yeast, Schizosaccharomyces pombe, has been extensively described by fluorescence microscopy. Here, we describe a large-scale, electron tomography investigation of S. pombe, including a 3D reconstruction of a complete eukaryotic cell volume at sufficient resolution to show both how many MTs there are in a bundle and their detailed architecture. Most cytoplasmic MTs are open at one end and capped at the other, providing evidence about their polarity. Electron-dense bridges between the MTs themselves and between MTs and the nuclear envelope were frequently observed. Finally, we have investigated structure/function relationships between MTs and both mitochondria and vesicles. Our analysis shows that electron tomography of well-preserved cells is ideally suited for describing fine ultrastructural details that were not visible with previous techniques.