Effect of ABO blood group mismatching on corneal epithelial cells: an in vitro study
Effect of ABO blood group mismatching on corneal epithelial cells: an in vitro study
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DOI：
10.1136/bjo.85.9.1104
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发表时间：
2001-09-01
影响因子：
4.1
通讯作者：
Harris, IM
中科院分区：
文献类型：
--
作者：
Chan, JH;Dua, HS;Harris, IM
目的-确定，在体外，血型ABO血型不匹配的角膜上皮细胞的影响。方法-角膜上皮细胞培养建立从32人尸体供体眼。将上皮细胞（每穆尔100穆尔4 × 10（2）个细胞）与抗血型抗原A、B和AB的抗体（有或没有补体）一起孵育4小时。使用Cytolite试剂通过荧光测定法测定细胞裂解。在闪烁计数器中计数孵育后剩余的活细胞。献血者的血型进行了回顾性测定，在一个盲目的marty.Results-Retrospective追踪献血者血型是可能的20个捐助者。在所有情况下，血型与细胞裂解试验所提示的血型一致。当已知A组细胞与抗A和抗AB抗体孵育时，观察到显著的细胞溶解，B组细胞与抗B和AB抗体孵育，AB组细胞与抗AB抗体孵育。溶解仅在补体存在下发生。用任何抗体均未观察到O组细胞的裂解。在所有情况下，溶解观察到只有与净（血清）抗体concentrations. Conclusions血型ABO血型不匹配的结果显着溶解角膜上皮细胞。裂解所需的抗体浓度等于血清中的浓度。在泪液和/或水溶液中不可能达到这样的抗体水平。这可能为血型匹配对角膜移植物影响的研究报告相互矛盾提供了解释。结果的可变性可能反映了进入角膜细胞的抗体水平，而不仅仅是错配。
Aim-To determine, in vitro, the effects of blood group ABO mismatching on corneal epithelial cells.Methods-Corneal epithelial cell cultures were established from 32 human cadaver donor eyes. Epithelial cells (100 mul of 4 X 10(2) cells per mul) were incubated for 4 hours with antibodies against blood group antigens A, B, and AB, with and without complement. Cell lysis was assayed by a chemiluminescent assay using Cytolite reagent. Live cells, remaining after incubation, were counted in a scintillation counter. The blood group of the donors was determined retrospectively, in a blinded manner.Results-Retrospective tracing of donor blood groups was possible for 20 donors. In all cases the blood group corresponded with that suggested by the cell lysis assay. Significant cell lysis was observed when known A group cells were incubated with anti-A and anti-AB antibody, B group cells were incubated with anti-B and AB antibody, and AB group cells were incubated with anti-AB antibody. Lysis occurred only in the presence of complement. No lysis of O group cells was observed with any of the antibodies. In all cases, lysis was observed only with neat (serum) antibody concentrations.Conclusions-Blood group ABO mismatching results in significant lysis of corneal epithelial cells. The antibody concentration required for lysis equals that found in serum. Such levels of antibody are unlikely to be achieved in tears and/or aqueous. This may offer an explanation for the conflicting reports of the studies on the effect of blood group matching on corneal grafts. The variability in the outcome may reflect the levels of antibodies gaining access to the corneal cells and not the mismatching alone.