The IbBBX24-IbTOE3-IbPRX17 module enhances abiotic stress tolerance by scavenging reactive oxygen species in sweet potato.
The IbBBX24-IbTOE3-IbPRX17 module enhances abiotic stress tolerance by scavenging reactive oxygen species in sweet potato.
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DOI：
10.1111/nph.17860
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发表时间：
2021-11
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通讯作者：
Huan Zhang;Zhen Wang;Xu Li;Xiao-Ru Gao;Zhuoru Dai;Yufei Cui;Yuhai Zhi;Qingchang Liu;H. Zhai;Shaopei Gao;Ning Zhao;Shaozhen He
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作者：
Huan Zhang;Zhen Wang;Xu Li;Xiao-Ru Gao;Zhuoru Dai;Yufei Cui;Yuhai Zhi;Qingchang Liu;H. Zhai;Shaopei Gao;Ning Zhao;Shaozhen He
土壤盐分和干旱限制了甘薯的产量。过氧化物酶（PRXs）对活性氧（ROS）的清除在植物逆境响应过程中是必不可少的，但在非生物胁迫下PRX的表达是如何调节的还不清楚。在这里，我们报告说，B-盒（BBX）家族转录因子IbBBX 24激活的III类过氧化物酶基因IbPRX 17的表达结合到其启动子。IbBBX 24和IbPRX 17的过表达显著提高了甘薯对盐和干旱胁迫的耐受性，而IbBBX 24的表达降低则增加了甘薯对盐和干旱胁迫的敏感性。在非生物胁迫下，IbBBX 24-和IbPRX 17-过表达株系表现出较高的过氧化物酶活性和较低的H2 O2积累相比，野生型。RNA测序分析显示IbBBX 24调节编码ROS清除酶（包括PRXs）的基因的表达。此外，IbBBX 24和APETALA 2（AP 2）蛋白IbTOE 3之间的相互作用增强IbBBX 24激活IbPRX 17转录的能力。过量表达IbTOE 3可通过清除活性氧提高烟草对盐和干旱胁迫的耐受性。总之，我们的研究结果阐明了IbBBX 24-IbTOE 3-IbPRX 17模块响应甘薯非生物胁迫的机制，并确定了开发具有增强的非生物胁迫耐受性的优良作物品种的候选基因。
Soil salinity and drought limit sweet potato yield. Scavenging of reactive oxygen species (ROS) by peroxidases (PRXs) is essential during plant stress responses, but how PRX expression is regulated under abiotic stress is not well understood. Here, we report that the B-box (BBX) family transcription factor IbBBX24 activates the expression of the class III peroxidase gene IbPRX17 by binding to its promoter. Overexpression of IbBBX24 and IbPRX17 significantly improved the tolerance of sweet potato to salt and drought stresses, while reducing IbBBX24 expression increased their susceptibility. Under abiotic stress, IbBBX24- and IbPRX17-overexpression lines showed higher peroxidase activity and lower H2 O2 accumulation compared to the wild type. RNA sequencing analysis revealed that IbBBX24 modulates the expression of genes encoding ROS scavenging enzymes, including PRXs. Moreover, interaction between IbBBX24 and the APETALA2 (AP2) protein IbTOE3 enhances the ability of IbBBX24 to activate IbPRX17 transcription. Overexpression of IbTOE3 improved the tolerance of tobacco plants to salt and drought stresses by scavenging ROS. Together, our findings elucidate the mechanism underlying the IbBBX24-IbTOE3-IbPRX17 module in response to abiotic stress in sweet potato and identify candidate genes for developing elite crop varieties with enhanced abiotic stress tolerance.